6u0t: Difference between revisions

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<SX load='6u0t' size='340' side='right' viewer='molstar' caption='[[6u0t]], [[Resolution|resolution]] 4.16&Aring;' scene=''>
<SX load='6u0t' size='340' side='right' viewer='molstar' caption='[[6u0t]], [[Resolution|resolution]] 4.16&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6u0t]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetth Tetth] and [http://en.wikipedia.org/wiki/Tetts Tetts]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U0T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U0T FirstGlance]. <br>
<table><tr><td colspan='2'>[[6u0t]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila] and [https://en.wikipedia.org/wiki/Tetrahymena_thermophila_SB210 Tetrahymena thermophila SB210]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U0T FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.16&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BTU1, BTU2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5911 TETTH]), TTHERM_00641119 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=312017 TETTS])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u0t OCA], [http://pdbe.org/6u0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u0t RCSB], [http://www.ebi.ac.uk/pdbsum/6u0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u0t ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u0t OCA], [https://pdbe.org/6u0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u0t RCSB], [https://www.ebi.ac.uk/pdbsum/6u0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u0t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TBA_TETTH TBA_TETTH]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB_TETTH TBB_TETTH]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
[https://www.uniprot.org/uniprot/TBA_TETTH TBA_TETTH] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present a near-atomic resolution map of the Tetrahymena doublet microtubule by cryoelectron microscopy. The map demonstrates that the network of microtubule inner proteins weaves into the tubulin lattice and forms an inner sheath. From mass spectrometry data and de novo modeling, we identified Rib43a proteins as the filamentous microtubule inner proteins in the protofilament ribbon region. The Rib43a-tubulin interaction leads to an elongated tubulin dimer distance every 2 dimers. In addition, the tubulin lattice structure with missing microtubule inner proteins (MIPs) by sarkosyl treatment shows significant longitudinal compaction and lateral angle change between protofilaments. These results are evidence that the MIPs directly affect and stabilize the tubulin lattice. It suggests that the doublet microtubule is an intrinsically stressed filament and that this stress could be manipulated in the regulation of ciliary waveforms.
 
Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins.,Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH Proc Natl Acad Sci U S A. 2019 Oct 1;116(40):19930-19938. doi:, 10.1073/pnas.1911119116. Epub 2019 Sep 16. PMID:31527277<ref>PMID:31527277</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6u0t" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Tetth]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetts]]
[[Category: Tetrahymena thermophila SB210]]
[[Category: Basu, K]]
[[Category: Basu K]]
[[Category: Bui, K H]]
[[Category: Bui KH]]
[[Category: Ichikawa, M]]
[[Category: Ichikawa M]]
[[Category: Khalifa, A A.Z]]
[[Category: Khalifa AAZ]]
[[Category: Vargas, J]]
[[Category: Vargas J]]
[[Category: Axoneme]]
[[Category: Cilia]]
[[Category: Doublet]]
[[Category: Microtubule inner protein]]
[[Category: Structural protein]]
Retrieved from "https://proteopedia.org/6u0t"