1cr9: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1cr9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CR9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1cr9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CR9 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr9 OCA], [https://pdbe.org/1cr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cr9 RCSB], [https://www.ebi.ac.uk/pdbsum/1cr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr9 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr9 OCA], [https://pdbe.org/1cr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cr9 RCSB], [https://www.ebi.ac.uk/pdbsum/1cr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr9 ProSAT]</span></td></tr>
</table>
</table>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cr9 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cr9 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The X-ray crystallographic structures of the anti-Syrian hamster prion protein (SHaPrP) monoclonal Fab 3F4 alone, as well as the complex with its cognate peptide epitope (SHaPrP 104-113), have been determined to atomic resolution. The conformation of the decapeptide is an Omega-loop. There are substantial alterations in the antibody combining region upon epitope binding. The peptide binds in a U-shaped groove on the Fab surface, with the two specificity determinants, Met109 and Met112, penetrating deeply into separate hydrophobic cavities formed by the heavy and light chain complementarity-determining regions. In addition to the numerous contacts between the Fab and the peptide, two intrapeptide hydrogen bonds are observed, perhaps indicating the structure bound to the Fab exists transiently in solution. This provides the first structural information on a portion of the PrP N-terminal region observed to be flexible in the NMR studies of SHPrP 90-231, SHaPrP 29-231 and mouse PrP 23-231. Antibody characterization of the antigenic surfaces of PrPC and PrPSc identifies this flexible region as a component of the conformational rearrangement that is an essential feature of prion disease.
Antibody binding defines a structure for an epitope that participates in the PrPC--&gt;PrPSc conformational change.,Kanyo ZF, Pan KM, Williamson RA, Burton DR, Prusiner SB, Fletterick RJ, Cohen FE J Mol Biol. 1999 Nov 5;293(4):855-63. PMID:10543972<ref>PMID:10543972</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1cr9" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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*[[Sandbox 20009|Sandbox 20009]]
*[[Sandbox 20009|Sandbox 20009]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Burton, D R]]
[[Category: Burton DR]]
[[Category: Cohen, F E]]
[[Category: Cohen FE]]
[[Category: Fletterick, R J]]
[[Category: Fletterick RJ]]
[[Category: Kanyo, Z F]]
[[Category: Kanyo ZF]]
[[Category: Pan, K M]]
[[Category: Pan KM]]
[[Category: Prusiner, S B]]
[[Category: Prusiner SB]]
[[Category: Williamson, R A]]
[[Category: Williamson RA]]
[[Category: Anti-prion antibody]]
[[Category: Fab 3f4]]
[[Category: Immune system]]

Revision as of 15:41, 13 March 2024

CRYSTAL STRUCTURE OF THE ANTI-PRION FAB 3F4

1cr9, resolution 2.00Å

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