8k9y: Difference between revisions

Latest revision as of 11:20, 14 March 2024

Crystal structure of Arabidopsis thaliana sulfotransferase SOT16 involved in glucosinolate biosynthesis

Structural highlights

8k9y is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.82Å
Ligands:	A3P, FLC, GOL, IPA
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SOT16_ARATH Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of desulfo-glucosinolates (dsGSs), the final step in the biosynthesis of the glucosinolate core structure. Substrate preference is desulfo-2-phenylethyl glucosinolate > desulfo-indol-3-yl methyl glucosinolate > desulfo-benzyl glucosinolate > desulfo-6-methylthiohexyl glucosinolate > desulfo-4-methylthiobutyl glucosinolate > desulfo-3-methylthiopropyl glucosinolate > desulfo-singrin > desulfo-3-butenyl glucosinolate.^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Piotrowski M, Schemenewitz A, Lopukhina A, Muller A, Janowitz T, Weiler EW, Oecking C. Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure. J Biol Chem. 2004 Dec 3;279(49):50717-25. Epub 2004 Sep 9. PMID:15358770 doi:https://dx.doi.org/10.1074/jbc.M407681200
↑ Hirai MY, Klein M, Fujikawa Y, Yano M, Goodenowe DB, Yamazaki Y, Kanaya S, Nakamura Y, Kitayama M, Suzuki H, Sakurai N, Shibata D, Tokuhisa J, Reichelt M, Gershenzon J, Papenbrock J, Saito K. Elucidation of gene-to-gene and metabolite-to-gene networks in arabidopsis by integration of metabolomics and transcriptomics. J Biol Chem. 2005 Jul 8;280(27):25590-5. Epub 2005 May 2. PMID:15866872 doi:https://dx.doi.org/M502332200
↑ Klein M, Reichelt M, Gershenzon J, Papenbrock J. The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed. FEBS J. 2006 Jan;273(1):122-36. PMID:16367753 doi:https://dx.doi.org/10.1111/j.1742-4658.2005.05048.x
↑ Klein M, Papenbrock J. Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana. Physiol Plant. 2009 Feb;135(2):140-9. doi: 10.1111/j.1399-3054.2008.01182.x. Epub, 2008 Dec 5. PMID:19077143 doi:https://dx.doi.org/10.1111/j.1399-3054.2008.01182.x
↑ Moldrup ME, Geu-Flores F, Olsen CE, Halkier BA. Modulation of sulfur metabolism enables efficient glucosinolate engineering. BMC Biotechnol. 2011 Jan 31;11:12. doi: 10.1186/1472-6750-11-12. PMID:21281472 doi:https://dx.doi.org/10.1186/1472-6750-11-12

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OCA

[1] Piotrowski M, Schemenewitz A, Lopukhina A, Muller A, Janowitz T, Weiler EW, Oecking C. Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure. J Biol Chem. 2004 Dec 3;279(49):50717-25. Epub 2004 Sep 9. PMID:15358770 doi:https://dx.doi.org/10.1074/jbc.M407681200

[2] Hirai MY, Klein M, Fujikawa Y, Yano M, Goodenowe DB, Yamazaki Y, Kanaya S, Nakamura Y, Kitayama M, Suzuki H, Sakurai N, Shibata D, Tokuhisa J, Reichelt M, Gershenzon J, Papenbrock J, Saito K. Elucidation of gene-to-gene and metabolite-to-gene networks in arabidopsis by integration of metabolomics and transcriptomics. J Biol Chem. 2005 Jul 8;280(27):25590-5. Epub 2005 May 2. PMID:15866872 doi:https://dx.doi.org/M502332200

[3] Klein M, Reichelt M, Gershenzon J, Papenbrock J. The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed. FEBS J. 2006 Jan;273(1):122-36. PMID:16367753 doi:https://dx.doi.org/10.1111/j.1742-4658.2005.05048.x

[4] Klein M, Papenbrock J. Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana. Physiol Plant. 2009 Feb;135(2):140-9. doi: 10.1111/j.1399-3054.2008.01182.x. Epub, 2008 Dec 5. PMID:19077143 doi:https://dx.doi.org/10.1111/j.1399-3054.2008.01182.x

[5] Moldrup ME, Geu-Flores F, Olsen CE, Halkier BA. Modulation of sulfur metabolism enables efficient glucosinolate engineering. BMC Biotechnol. 2011 Jan 31;11:12. doi: 10.1186/1472-6750-11-12. PMID:21281472 doi:https://dx.doi.org/10.1186/1472-6750-11-12

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8k9y is ON HOLD
+==Crystal structure of Arabidopsis thaliana sulfotransferase SOT16 involved in glucosinolate biosynthesis==
+<StructureSection load='8k9y' size='340' side='right'caption='[[8k9y]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8k9y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8K9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8K9Y FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8k9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8k9y OCA], [https://pdbe.org/8k9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8k9y RCSB], [https://www.ebi.ac.uk/pdbsum/8k9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8k9y ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SOT16_ARATH SOT16_ARATH] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of desulfo-glucosinolates (dsGSs), the final step in the biosynthesis of the glucosinolate core structure. Substrate preference is desulfo-2-phenylethyl glucosinolate > desulfo-indol-3-yl methyl glucosinolate > desulfo-benzyl glucosinolate > desulfo-6-methylthiohexyl glucosinolate > desulfo-4-methylthiobutyl glucosinolate > desulfo-3-methylthiopropyl glucosinolate > desulfo-singrin > desulfo-3-butenyl glucosinolate.<ref>PMID:15358770</ref> <ref>PMID:15866872</ref> <ref>PMID:16367753</ref> <ref>PMID:19077143</ref> <ref>PMID:21281472</ref>
-Authors:
+==See Also==
+*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]]
-Description:
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Iwamoto Y]]
+[[Category: Kakuta Y]]
+[[Category: Saito S]]
+[[Category: Teramoto T]]

8k9y: Difference between revisions

Latest revision as of 11:20, 14 March 2024

Crystal structure of Arabidopsis thaliana sulfotransferase SOT16 involved in glucosinolate biosynthesis

Structural highlights

Function

See Also

References

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