3q1s: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 3: Line 3:
<StructureSection load='3q1s' size='340' side='right'caption='[[3q1s]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
<StructureSection load='3q1s' size='340' side='right'caption='[[3q1s]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3q1s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q1S FirstGlance]. <br>
<table><tr><td colspan='2'>[[3q1s]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q1S FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fn0|3fn0]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IL22, ILTIF, ZCYTO18, UNQ3099/PRO10096 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1s OCA], [https://pdbe.org/3q1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q1s RCSB], [https://www.ebi.ac.uk/pdbsum/3q1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1s ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1s OCA], [https://pdbe.org/3q1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q1s RCSB], [https://www.ebi.ac.uk/pdbsum/3q1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1s ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/IL22_HUMAN IL22_HUMAN]] Cytokine that contributes to the inflammatory response in vivo.  
[https://www.uniprot.org/uniprot/IL22_HUMAN IL22_HUMAN] Cytokine that contributes to the inflammatory response in vivo.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Antibody Z13e1 is a relatively broadly neutralizing anti-human immunodeficiency virus type 1 antibody that recognizes the membrane-proximal external region (MPER) of the human immunodeficiency virus type 1 envelope glycoprotein gp41. Based on the crystal structure of an MPER epitope peptide in complex with Z13e1 Fab, we identified an unrelated protein, interleukin (IL)-22, with a surface-exposed region that is structurally homologous in its backbone to the gp41 Z13e1 epitope. By grafting the gp41 Z13e1 epitope sequence onto the structurally homologous region in IL-22, we engineered a novel protein (Z13-IL22-2) that contains the MPER epitope sequence for use as a potential immunogen and as a reagent for the detection of Z13e1-like antibodies. The Z13-IL22-2 protein binds Fab Z13e1 with a K(d) of 73 nM. The crystal structure of Z13-IL22-2 in complex with Fab Z13e1 shows that the epitope region is faithfully replicated in the Fab-bound scaffold protein; however, isothermal calorimetry studies indicate that Fab binding to Z13-IL22-2 is not a lock-and-key event, leaving open the question of whether conformational changes upon binding occur in the Fab, in Z13-IL-22, or in both.
 
Structure-Based Design of a Protein Immunogen that Displays an HIV-1 gp41 Neutralizing Epitope.,Stanfield RL, Julien JP, Pejchal R, Gach JS, Zwick MB, Wilson IA J Mol Biol. 2011 Dec 2;414(3):460-76. Epub 2011 Oct 15. PMID:22033480<ref>PMID:22033480</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3q1s" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Interleukin 3D structures|Interleukin 3D structures]]
*[[Interleukin 3D structures|Interleukin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Gach, J S]]
[[Category: Gach JS]]
[[Category: Julien, J P]]
[[Category: Julien J-P]]
[[Category: Pejchal, R]]
[[Category: Pejchal R]]
[[Category: Stanfield, R L]]
[[Category: Stanfield RL]]
[[Category: Wilson, I A]]
[[Category: Wilson IA]]
[[Category: Zwick, M B]]
[[Category: Zwick MB]]
[[Category: Antibody]]
[[Category: Gp41]]
[[Category: Immune system-cytokine complex]]
[[Category: Immunoglobulin fold]]

Revision as of 11:30, 14 March 2024

HIV-1 neutralizing antibody Z13e1 in complex with epitope display protein

3q1s, resolution 2.15Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=3q1s&oldid=4095500"