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<StructureSection load='3r6w' size='340' side='right'caption='[[3r6w]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
<StructureSection load='3r6w' size='340' side='right'caption='[[3r6w]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3r6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R6W FirstGlance]. <br>
<table><tr><td colspan='2'>[[3r6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R6W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NFZ:NITROFURAZONE'>NFZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.085&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2v9c|2v9c]], [[3lt5|3lt5]], [[3keg|3keg]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NFZ:NITROFURAZONE'>NFZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">azoR1, PA0785 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r6w OCA], [https://pdbe.org/3r6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r6w RCSB], [https://www.ebi.ac.uk/pdbsum/3r6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r6w ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r6w OCA], [https://pdbe.org/3r6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r6w RCSB], [https://www.ebi.ac.uk/pdbsum/3r6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r6w ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/AZOR1_PSEAE AZOR1_PSEAE]] Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity (By similarity).  
[https://www.uniprot.org/uniprot/AZOR1_PSEAE AZOR1_PSEAE] Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Azoreductases are well known for azo pro-drug activation by gut flora. We show that azoreductases have a wider role in drug metabolism than previously thought as they can also reduce and hence activate nitrofurazone. Nitrofurazone, a nitroaromatic drug, is a broad spectrum antibiotic which has until now been considered as activated in bacteria by nitroreductases. The structure of the azoreductase with nitrofurazone bound was solved at 2.08 A and shows nitrofurazone in an active conformation. Based on the structural information, the kinetics and stoichiometry of nitrofurazone reduction by azoreductase from P. aeruginosa, we propose a mechanism of activation which accounts for the ability of azoreductases to reduce both azo and nitroaromatic drugs. This mode of activation can explain the cytotoxic side-effects of nitrofurazone through human azoreductase homologues.
 
Activation of nitrofurazone by azoreductases: multiple activities in one enzyme.,Ryan A, Kaplan E, Laurieri N, Lowe E, Sim E Sci Rep. 2011;1:63. Epub 2011 Aug 12. PMID:22355582<ref>PMID:22355582</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3r6w" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Kaplan, K]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Laurieri, N]]
[[Category: Kaplan K]]
[[Category: Lowe, E]]
[[Category: Laurieri N]]
[[Category: Ryan, A]]
[[Category: Lowe E]]
[[Category: Sim, E]]
[[Category: Ryan A]]
[[Category: Azoreductase]]
[[Category: Sim E]]
[[Category: Nitrofurazone]]
[[Category: Nitroreductase]]
[[Category: Oxidoreductase]]
[[Category: P. aeruginosa]]
[[Category: Short-flavodoxin]]

Latest revision as of 12:12, 14 March 2024

paAzoR1 binding to nitrofurazone

3r6w, resolution 2.08Å

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