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<StructureSection load='3uby' size='340' side='right'caption='[[3uby]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3uby' size='340' side='right'caption='[[3uby]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3uby]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UBY FirstGlance]. <br>
<table><tr><td colspan='2'>[[3uby]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UBY FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDC:N3,N4-ETHENO-2-DEOXYCYTIDINE-5-MONOPHOSPHATE'>EDC</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qi5|3qi5]], [[1ewn|1ewn]], [[1f4r|1f4r]], [[1f6o|1f6o]], [[1bnk|1bnk]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDC:N3,N4-ETHENO-2-DEOXYCYTIDINE-5-MONOPHOSPHATE'>EDC</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MPG, AAG, ANPG, MID1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uby OCA], [https://pdbe.org/3uby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uby RCSB], [https://www.ebi.ac.uk/pdbsum/3uby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uby ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uby OCA], [https://pdbe.org/3uby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uby RCSB], [https://www.ebi.ac.uk/pdbsum/3uby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uby ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/3MG_HUMAN 3MG_HUMAN]] Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.  
[https://www.uniprot.org/uniprot/3MG_HUMAN 3MG_HUMAN] Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To efficiently repair DNA, human alkyladenine DNA glycosylase (AAG) must search the million-fold excess of unmodified DNA bases to find a handful of DNA lesions. Such a search can be facilitated by the ability of glycosylases, like AAG, to interact with DNA using two affinities: a lower-affinity interaction in a searching process and a higher-affinity interaction for catalytic repair. Here, we present crystal structures of AAG trapped in two DNA-bound states. The lower-affinity depiction allows us to investigate, for the first time, the conformation of this protein in the absence of a tightly bound DNA adduct. We find that active site residues of AAG involved in binding lesion bases are in a disordered state. Furthermore, two loops that contribute significantly to the positive electrostatic surface of AAG are disordered. Additionally, a higher-affinity state of AAG captured here provides a fortuitous snapshot of how this enzyme interacts with a DNA adduct that resembles a one-base loop.
 
Searching for DNA Lesions: Structural Evidence for Lower- and Higher-Affinity DNA Binding Conformations of Human Alkyladenine DNA Glycosylase.,Setser JW, Lingaraju GM, Davis CA, Samson LD, Drennan CL Biochemistry. 2011 Dec 20. PMID:22148158<ref>PMID:22148158</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3uby" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: DNA-3-methyladenine glycosylase II]]
[[Category: Homo sapiens]]
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Davis, C A]]
[[Category: Davis CA]]
[[Category: Drennan, C L]]
[[Category: Drennan CL]]
[[Category: Lingaraju, G M]]
[[Category: Lingaraju GM]]
[[Category: Samson, L D]]
[[Category: Samson LD]]
[[Category: Setser, J W]]
[[Category: Setser JW]]
[[Category: Aag]]
[[Category: Alkyladenine dna glycosylase fold]]
[[Category: Dna binding]]
[[Category: Dna repair]]
[[Category: Hydrolase-dna complex]]
[[Category: Nucleus]]

Latest revision as of 13:54, 14 March 2024

Crystal structure of human alklyadenine DNA glycosylase in a lower and higher-affinity complex with DNA

3uby, resolution 2.00Å

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