4h0p: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4h0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptococcus_neoformans Cryptococcus neoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H0P FirstGlance]. <br>
<table><tr><td colspan='2'>[[4h0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptococcus_neoformans Cryptococcus neoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H0P FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0p OCA], [https://pdbe.org/4h0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h0p RCSB], [https://www.ebi.ac.uk/pdbsum/4h0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0p ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.894&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0p OCA], [https://pdbe.org/4h0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h0p RCSB], [https://www.ebi.ac.uk/pdbsum/4h0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0p ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/J9W3A6_CRYNH J9W3A6_CRYNH]  
[https://www.uniprot.org/uniprot/J9W3A6_CRYNH J9W3A6_CRYNH]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Acetate kinases (ACKs) are members of the acetate and sugar kinase/hsp70/actin (ASKHA) superfamily and catalyze the reversible phosphorylation of acetate, with ADP/ATP the most common phosphoryl acceptor/donor. While prokaryotic ACKs have been the subject of extensive biochemical and structural characterization, there is a comparative paucity of information on eukaryotic ACKs, and prior to this report, no structure of an ACK of eukaryotic origin was available. We determined the structures of ACKs from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans. Each active site is located at an interdomain interface, and the acetate and phosphate binding pockets display sequence and structural conservation with their prokaryotic counterparts. Interestingly, the E. histolytica ACK has previously been shown to be pyrophosphate (PP(i))-dependent, and is the first ACK demonstrated to have this property. Examination of its structure demonstrates how subtle amino acid substitutions within the active site have converted cosubstrate specificity from ATP to PP(i) while retaining a similar backbone conformation. Differences in the angle between domains surrounding the active site suggest that interdomain movement may accompany catalysis. Taken together, these structures are consistent with the eukaryotic ACKs following a similar reaction mechanism as is proposed for the prokaryotic homologs.
Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans.,Thaker TM, Tanabe M, Fowler ML, Preininger AM, Ingram-Smith C, Smith KS, Iverson TM J Struct Biol. 2012 Nov 16. pii: S1047-8477(12)00313-9. doi:, 10.1016/j.jsb.2012.11.001. PMID:23159802<ref>PMID:23159802</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4h0p" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 16:01, 14 March 2024

Crystal Structure of Acetate Kinase from Cryptococcus neoformans

4h0p, resolution 1.89Å

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