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<StructureSection load='3del' size='340' side='right'caption='[[3del]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
<StructureSection load='3del' size='340' side='right'caption='[[3del]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3del]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"chlamydozoon_trachomatis"_(busacca_1935)_moshkovski_1945 "chlamydozoon trachomatis" (busacca 1935) moshkovski 1945]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DEL FirstGlance]. <br>
<table><tr><td colspan='2'>[[3del]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DEL FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hsl|1hsl]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">artJ, CT_381 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=813 "Chlamydozoon trachomatis" (Busacca 1935) Moshkovski 1945])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3del FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3del OCA], [https://pdbe.org/3del PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3del RCSB], [https://www.ebi.ac.uk/pdbsum/3del PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3del ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3del FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3del OCA], [https://pdbe.org/3del PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3del RCSB], [https://www.ebi.ac.uk/pdbsum/3del PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3del ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/ARTJ_CHLTR ARTJ_CHLTR]] Probably part of an ABC transporter complex involved in arginine transport. Binds arginine (By similarity). Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection.<ref>PMID:20592031</ref>
[https://www.uniprot.org/uniprot/ARTJ_CHLTR ARTJ_CHLTR] Probably part of an ABC transporter complex involved in arginine transport. Binds arginine (By similarity). Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection.<ref>PMID:20592031</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3del ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3del ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We present an interdisciplinary approach that, by incorporating a range of experimental and computational techniques, allows the identification and characterization of functional/immunogenic domains. This approach has been applied to ArtJ, an arginine binding protein whose orthologs in Chlamydiae trachomatis (CT ArtJ) and pneumoniae (CPn ArtJ) are shown to have different immunogenic properties despite a high sequence similarity (60% identity). We have solved the crystallographic structures of CT ArtJ and CPn ArtJ, which are found to display a type-II transporter fold organized in two alpha-beta domains with the arginine-binding region at their interface. Although ArtJ is considered to belong to the periplasm, we found that both domains contain regions exposed on the bacterial surface. Moreover, we show that recombinant ArtJ binds to epithelial cells in vitro, suggesting a role for ArtJ in host-cell adhesion during Chlamydia infection. Experimental epitope mapping and computational analysis of physico-chemical determinants of antibody recognition revealed that immunogenic epitopes reside mainly in the terminal (D1) domain of both CPn and CT ArtJ, while the surface properties of the respective binding-prone regions appear sufficiently different to assume divergent immunogenic behavior. Neutralization assays revealed that sera raised against CPn ArtJ D1 partially reduce both CPn and CT infectivity in vitro, suggesting that functional antibodies directed against this domain may potentially impair chlamydial infectivity. These findings suggest that the approach presented here, combining functional and structure-based analyses of evolutionary-related antigens can be a valuable tool for the identification of cross-species immunogenic epitopes for vaccine development.
Exploiting antigenic diversity for vaccine design: the Chlamydia ArtJ paradigm.,Soriani M, Petit P, Grifantini R, Petracca R, Gancitano G, Frigimelica E, Nardelli F, Garcia C, Spinelli S, Scarabelli G, Fiorucci S, Affentranger R, Ferrer-Navarro M, Zacharias M, Colombo G, Vuillard L, Daura X, Grandi G J Biol Chem. 2010 Jun 30. PMID:20592031<ref>PMID:20592031</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3del" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chlamydia trachomatis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Petit, P]]
[[Category: Petit P]]
[[Category: Spinelli, S]]
[[Category: Spinelli S]]
[[Category: Vuillard, L]]
[[Category: Vuillard L]]
[[Category: Arginine binding]]
[[Category: Periplasmic protein]]
[[Category: Protein binding]]
[[Category: Transport protein]]

Latest revision as of 08:23, 20 March 2024

The structure of CT381, the arginine binding protein from the periplasm Chlamydia trachomatis

3del, resolution 1.92Å

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