3gyw: Difference between revisions

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<StructureSection load='3gyw' size='340' side='right'caption='[[3gyw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='3gyw' size='340' side='right'caption='[[3gyw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3gyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GYW FirstGlance]. <br>
<table><tr><td colspan='2'>[[3gyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GYW FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3fs3|3fs3]], [[3gyv|3gyv]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pfl0185c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gyw OCA], [https://pdbe.org/3gyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gyw RCSB], [https://www.ebi.ac.uk/pdbsum/3gyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gyw ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gyw OCA], [https://pdbe.org/3gyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gyw RCSB], [https://www.ebi.ac.uk/pdbsum/3gyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gyw ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8I608_PLAF7 Q8I608_PLAF7]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gyw ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gyw ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of Plasmodium falciparum nucleosome assembly protein (PfNapL) was determined by iodide-SAD/SIRAS phasing methods using iodide-SAD data to 3.0 A resolution and native data to 2.4 A resolution. Halide-derivatized PfNapL crystals were obtained using the quick cryo-soaking method in which the native crystals were soaked in a cryosolution consisting of 500 mM NaI for a short period of 30-60 s and data were collected at an in-house X-ray source using Cu Kalpha radiation. Despite a low anomalous signal-to-noise ratio of &lt;1.2 in the &gt;3.5 A resolution bin, the data were sufficient to determine the structure by SAD/SIR/SIRAS methods using the soaked iodides. Previously, structure solution had failed with both molecular-replacement and selenomethionine-derivatization techniques owing to reasons that are detailed in this work. The phasing at low resolution with three iodides per monomer with high temperature factors was successful using any of the SAD, SIR or SIRAS methods.
Iodide-SAD, SIR and SIRAS phasing for structure solution of a nucleosome assembly protein.,Yogavel M, Gill J, Sharma A Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):618-22. Epub 2009, May 15. PMID:19465776<ref>PMID:19465776</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3gyw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Plaf7]]
[[Category: Plasmodium falciparum 3D7]]
[[Category: Gill, J]]
[[Category: Gill J]]
[[Category: Sharma, A]]
[[Category: Sharma A]]
[[Category: Yogavel, M]]
[[Category: Yogavel M]]
[[Category: Chaperone]]
[[Category: Histone recognition]]
[[Category: Nucleosome assembly protein]]
[[Category: Sir]]
[[Category: Sira]]

Latest revision as of 08:26, 20 March 2024

Crystal structure of nucleosome assembly protein from Plasmodium falciparum at 2.4 A resolution

3gyw, resolution 2.40Å

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