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| ==Crystal structure of NtcA in apo-form== | | ==Crystal structure of NtcA in apo-form== |
| <StructureSection load='3la7' size='340' side='right' caption='[[3la7]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='3la7' size='340' side='right'caption='[[3la7]], [[Resolution|resolution]] 1.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3la7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LA7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LA7 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3la7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LA7 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3la2|3la2]], [[3la3|3la3]]</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">alr4392, bifA, ntcA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3la7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3la7 OCA], [https://pdbe.org/3la7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3la7 RCSB], [https://www.ebi.ac.uk/pdbsum/3la7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3la7 ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3la7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3la7 OCA], [http://pdbe.org/3la7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3la7 RCSB], [http://www.ebi.ac.uk/pdbsum/3la7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3la7 ProSAT]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/NTCA_NOSS1 NTCA_NOSS1]] Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control. Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3'. | | [https://www.uniprot.org/uniprot/NTCA_NOSS1 NTCA_NOSS1] Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control. Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3'. |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3la7 ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3la7 ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| 2-oxogluatarate (2-OG), a metabolite of the highly conserved Krebs cycle, not only plays a critical role in metabolism, but also constitutes a signaling molecule in a variety of organisms ranging from bacteria to plants and animals. In cyanobacteria, the accumulation of 2-OG constitutes the signal of nitrogen starvation and NtcA, a global transcription factor, has been proposed as a putative receptor for 2-OG. Here we present three crystal structures of NtcA from the cyanobacterium Anabaena: the apoform, and two ligand-bound forms in complex with either 2-OG or its analogue 2,2-difluoropentanedioic acid. All structures assemble as homodimers, with each subunit composed of an N-terminal effector-binding domain and a C-terminal DNA-binding domain connected by a long helix (C-helix). The 2-OG binds to the effector-binding domain at a pocket similar to that used by cAMP in catabolite activator protein, but with a different pattern. Comparative structural analysis reveals a putative signal transmission route upon 2-OG binding. A tighter coiled-coil conformation of the two C-helices induced by 2-OG is crucial to maintain the proper distance between the two F-helices for DNA recognition. Whereas catabolite activator protein adopts a transition from off-to-on state upon cAMP binding, our structural analysis explains well why NtcA can bind to DNA even in its apoform, and how 2-OG just enhances the DNA-binding activity of NtcA. These findings provided the structural insights into the function of a global transcription factor regulated by 2-OG, a metabolite standing at a crossroad between carbon and nitrogen metabolisms.
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| Structural basis for the allosteric control of the global transcription factor NtcA by the nitrogen starvation signal 2-oxoglutarate.,Zhao MX, Jiang YL, He YX, Chen YF, Teng YB, Chen Y, Zhang CC, Zhou CZ Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12487-92. Epub 2010 Jun 28. PMID:20616047<ref>PMID:20616047</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3la7" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Anabaena 7120]] | | [[Category: Large Structures]] |
| [[Category: Chen, Y F]] | | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] |
| [[Category: Chen, Y X]] | | [[Category: Chen YF]] |
| [[Category: He, Y X]] | | [[Category: Chen YX]] |
| [[Category: Jiang, Y L]] | | [[Category: He YX]] |
| [[Category: Teng, Y B]] | | [[Category: Jiang YL]] |
| [[Category: Zhang, C C]] | | [[Category: Teng YB]] |
| [[Category: Zhao, M X]] | | [[Category: Zhang CC]] |
| [[Category: Zhou, C Z]] | | [[Category: Zhao MX]] |
| [[Category: Activator]]
| | [[Category: Zhou CZ]] |
| [[Category: Dna-binding]]
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| [[Category: Nitrogen regulator]]
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| [[Category: Transcription]]
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| [[Category: Transcription regulation]]
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