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==Crystal structure of a Major Facilitator Superfamily (MFS) transporter, FucP, in the outward conformation==
==Crystal structure of a Major Facilitator Superfamily (MFS) transporter, FucP, in the outward conformation==
<StructureSection load='3o7q' size='340' side='right' caption='[[3o7q]], [[Resolution|resolution]] 3.14&Aring;' scene=''>
<StructureSection load='3o7q' size='340' side='right'caption='[[3o7q]], [[Resolution|resolution]] 3.14&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3o7q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O7Q FirstGlance]. <br>
<table><tr><td colspan='2'>[[3o7q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O7Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O7Q FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.143&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3o7p|3o7p]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fucP, b2801, JW2772 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7q OCA], [https://pdbe.org/3o7q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o7q RCSB], [https://www.ebi.ac.uk/pdbsum/3o7q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7q ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o7q OCA], [http://pdbe.org/3o7q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o7q RCSB], [http://www.ebi.ac.uk/pdbsum/3o7q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o7q ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/FUCP_ECOLI FUCP_ECOLI]] Mediates the uptake of L-fucose across the boundary membrane with the concomitant transport of protons into the cell (symport system). Can also transport L-galactose and D-arabinose, but at reduced rates compared with L-fucose. Is not able to transport L-rhamnose and L-arabinose.<ref>PMID:2829831</ref>
[https://www.uniprot.org/uniprot/FUCP_ECOLI FUCP_ECOLI] Mediates the uptake of L-fucose across the boundary membrane with the concomitant transport of protons into the cell (symport system). Can also transport L-galactose and D-arabinose, but at reduced rates compared with L-fucose. Is not able to transport L-rhamnose and L-arabinose.<ref>PMID:2829831</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3o7q ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3o7q ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The major facilitator superfamily (MFS) transporters are an ancient and widespread family of secondary active transporters. In Escherichia coli, the uptake of l-fucose, a source of carbon for microorganisms, is mediated by an MFS proton symporter, FucP. Despite intensive study of the MFS transporters, atomic structure information is only available on three proteins and the outward-open conformation has yet to be captured. Here we report the crystal structure of FucP at 3.1 A resolution, which shows that it contains an outward-open, amphipathic cavity. The similarly folded amino and carboxyl domains of FucP have contrasting surface features along the transport path, with negative electrostatic potential on the N domain and hydrophobic surface on the C domain. FucP only contains two acidic residues along the transport path, Asp 46 and Glu 135, which can undergo cycles of protonation and deprotonation. Their essential role in active transport is supported by both in vivo and in vitro experiments. Structure-based biochemical analyses provide insights into energy coupling, substrate recognition and the transport mechanism of FucP.
Structure of a fucose transporter in an outward-open conformation.,Dang S, Sun L, Huang Y, Lu F, Liu Y, Gong H, Wang J, Yan N Nature. 2010 Oct 7;467(7316):734-8. Epub 2010 Sep 26. PMID:20877283<ref>PMID:20877283</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3o7q" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Lactose Permease|Lactose Permease]]
*[[Lactose Permease|Lactose Permease]]
*[[Symporter|Symporter]]
*[[Symporter 3D structures|Symporter 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Dang, S Y]]
[[Category: Large Structures]]
[[Category: Sun, L F]]
[[Category: Dang SY]]
[[Category: Wang, J]]
[[Category: Sun LF]]
[[Category: Yan, N]]
[[Category: Wang J]]
[[Category: L-fucose]]
[[Category: Yan N]]
[[Category: Multi-pass membrane protein]]
[[Category: Symporter]]
[[Category: Transport protein]]
[[Category: Transporter]]
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