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<StructureSection load='3wo6' size='340' side='right'caption='[[3wo6]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='3wo6' size='340' side='right'caption='[[3wo6]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3wo6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bachd Bachd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WO6 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3wo6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_halodurans_C-125 Alkalihalobacillus halodurans C-125]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WO6 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.403&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wo7|3wo7]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yidC2, BH1169 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272558 BACHD])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wo6 OCA], [https://pdbe.org/3wo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wo6 RCSB], [https://www.ebi.ac.uk/pdbsum/3wo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wo6 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wo6 OCA], [https://pdbe.org/3wo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wo6 RCSB], [https://www.ebi.ac.uk/pdbsum/3wo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wo6 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/YIDC2_BACHD YIDC2_BACHD]] Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins (By similarity).  
[https://www.uniprot.org/uniprot/YIDC2_HALH5 YIDC2_HALH5] Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins.[HAMAP-Rule:MF_01811]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Newly synthesized membrane proteins must be accurately inserted into the membrane, folded and assembled for proper functioning. The protein YidC inserts its substrates into the membrane, thereby facilitating membrane protein assembly in bacteria; the homologous proteins Oxa1 and Alb3 have the same function in mitochondria and chloroplasts, respectively. In the bacterial cytoplasmic membrane, YidC functions as an independent insertase and a membrane chaperone in cooperation with the translocon SecYEG. Here we present the crystal structure of YidC from Bacillus halodurans, at 2.4 A resolution. The structure reveals a novel fold, in which five conserved transmembrane helices form a positively charged hydrophilic groove that is open towards both the lipid bilayer and the cytoplasm but closed on the extracellular side. Structure-based in vivo analyses reveal that a conserved arginine residue in the groove is important for the insertion of membrane proteins by YidC. We propose an insertion mechanism for single-spanning membrane proteins, in which the hydrophilic environment generated by the groove recruits the extracellular regions of substrates into the low-dielectric environment of the membrane.
 
Structural basis of Sec-independent membrane protein insertion by YidC.,Kumazaki K, Chiba S, Takemoto M, Furukawa A, Nishiyama K, Sugano Y, Mori T, Dohmae N, Hirata K, Nakada-Nakura Y, Maturana AD, Tanaka Y, Mori H, Sugita Y, Arisaka F, Ito K, Ishitani R, Tsukazaki T, Nureki O Nature. 2014 May 22;509(7501):516-20. doi: 10.1038/nature13167. Epub 2014 Apr 16. PMID:24739968<ref>PMID:24739968</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wo6" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Insertase|Insertase]]
*[[Insertase|Insertase]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bachd]]
[[Category: Alkalihalobacillus halodurans C-125]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Ishitani, R]]
[[Category: Ishitani R]]
[[Category: Kumazaki, K]]
[[Category: Kumazaki K]]
[[Category: Nureki, O]]
[[Category: Nureki O]]
[[Category: Tsukazaki, T]]
[[Category: Tsukazaki T]]
[[Category: Alpha helical]]
[[Category: Membrane protein]]

Latest revision as of 08:46, 20 March 2024

Crystal structure of YidC from Bacillus halodurans (form I)

3wo6, resolution 2.40Å

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