5eo7: Difference between revisions

Latest revision as of 09:11, 20 March 2024

Crystal structure of AOL

Structural highlights

5eo7 is a 3 chain structure with sequence from Aspergillus oryzae RIB40. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	SFU, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LECF_ASPOR Lectin that specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid (PubMed:12092808, PubMed:27318092, PubMed:28041800). Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains (PubMed:17383961, PubMed:19109923). Binds to fucose residues of IgE in mice and human, causing antigen-independent IgE-mediated mast cell activation and anaphylactoid reactions in mice and is possibly implicated in allergic response to Aspergillus oryzae in humans (PubMed:21790704). Induces secretion of pro-inflammatory cytokines IL6 and IL8 implicated in ocular diseases such as mycotic keratitis, probably through its interaction with host toll-like receptors TLR2 and TLR4, followed by up-regulation of pro-inflammatory cytokines (PubMed:28470344).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Ishida H, Moritani T, Hata Y, Kawato A, Suginami K, Abe Y, Imayasu S. Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae. Biosci Biotechnol Biochem. 2002 May;66(5):1002-8. PMID:12092808 doi:10.1271/bbb.66.1002
↑ Matsumura K, Higashida K, Ishida H, Hata Y, Yamamoto K, Shigeta M, Mizuno-Horikawa Y, Wang X, Miyoshi E, Gu J, Taniguchi N. Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose. J Biol Chem. 2007 May 25;282(21):15700-8. PMID:17383961 doi:10.1074/jbc.M701195200
↑ Matsumura K, Higashida K, Hata Y, Kominami J, Nakamura-Tsuruta S, Hirabayashi J. Comparative analysis of oligosaccharide specificities of fucose-specific lectins from Aspergillus oryzae and Aleuria aurantia using frontal affinity chromatography. Anal Biochem. 2009 Mar 15;386(2):217-21. PMID:19109923 doi:10.1016/j.ab.2008.11.044
↑ Yamaki K, Yoshino S. Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE. Scand J Immunol. 2011 Nov;74(5):445-53. PMID:21790704 doi:10.1111/j.1365-3083.2011.02598.x
↑ Makyio H, Shimabukuro J, Suzuki T, Imamura A, Ishida H, Kiso M, Ando H, Kato R. Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin. Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30987-1. doi:, 10.1016/j.bbrc.2016.06.069. PMID:27318092 doi:https://dx.doi.org/10.1016/j.bbrc.2016.06.069
↑ Shimabukuro J, Makyio H, Suzuki T, Nishikawa Y, Kawasaki M, Imamura A, Ishida H, Ando H, Kato R, Kiso M. Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing. Bioorg Med Chem. 2016 Dec 19. pii: S0968-0896(16)31172-5. doi:, 10.1016/j.bmc.2016.12.021. PMID:28041800 doi:https://dx.doi.org/10.1016/j.bmc.2016.12.021
↑ Ballal S, Belur S, Laha P, Roy S, Swamy BM, Inamdar SR. Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host-pathogen interactions leading to mycotic keratitis. Mol Cell Biochem. 2017 Oct;434(1-2):209-219. PMID:28470344 doi:10.1007/s11010-017-3050-9

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OCA

[1] Ishida H, Moritani T, Hata Y, Kawato A, Suginami K, Abe Y, Imayasu S. Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae. Biosci Biotechnol Biochem. 2002 May;66(5):1002-8. PMID:12092808 doi:10.1271/bbb.66.1002

[2] Matsumura K, Higashida K, Ishida H, Hata Y, Yamamoto K, Shigeta M, Mizuno-Horikawa Y, Wang X, Miyoshi E, Gu J, Taniguchi N. Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose. J Biol Chem. 2007 May 25;282(21):15700-8. PMID:17383961 doi:10.1074/jbc.M701195200

[3] Matsumura K, Higashida K, Hata Y, Kominami J, Nakamura-Tsuruta S, Hirabayashi J. Comparative analysis of oligosaccharide specificities of fucose-specific lectins from Aspergillus oryzae and Aleuria aurantia using frontal affinity chromatography. Anal Biochem. 2009 Mar 15;386(2):217-21. PMID:19109923 doi:10.1016/j.ab.2008.11.044

[4] Yamaki K, Yoshino S. Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE. Scand J Immunol. 2011 Nov;74(5):445-53. PMID:21790704 doi:10.1111/j.1365-3083.2011.02598.x

[5] Makyio H, Shimabukuro J, Suzuki T, Imamura A, Ishida H, Kiso M, Ando H, Kato R. Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin. Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30987-1. doi:, 10.1016/j.bbrc.2016.06.069. PMID:27318092 doi:https://dx.doi.org/10.1016/j.bbrc.2016.06.069

[6] Shimabukuro J, Makyio H, Suzuki T, Nishikawa Y, Kawasaki M, Imamura A, Ishida H, Ando H, Kato R, Kiso M. Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing. Bioorg Med Chem. 2016 Dec 19. pii: S0968-0896(16)31172-5. doi:, 10.1016/j.bmc.2016.12.021. PMID:28041800 doi:https://dx.doi.org/10.1016/j.bmc.2016.12.021

[7] Ballal S, Belur S, Laha P, Roy S, Swamy BM, Inamdar SR. Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host-pathogen interactions leading to mycotic keratitis. Mol Cell Biochem. 2017 Oct;434(1-2):209-219. PMID:28470344 doi:10.1007/s11010-017-3050-9

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[2]

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[5]

[6]

[7]

@@ Line 10: / Line 10: @@
 == Function ==
 [https://www.uniprot.org/uniprot/LECF_ASPOR LECF_ASPOR] Lectin that specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid (PubMed:12092808, PubMed:27318092, PubMed:28041800). Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains (PubMed:17383961, PubMed:19109923). Binds to fucose residues of IgE in mice and human, causing antigen-independent IgE-mediated mast cell activation and anaphylactoid reactions in mice and is possibly implicated in allergic response to Aspergillus oryzae in humans (PubMed:21790704). Induces secretion of pro-inflammatory cytokines IL6 and IL8 implicated in ocular diseases such as mycotic keratitis, probably through its interaction with host toll-like receptors TLR2 and TLR4, followed by up-regulation of pro-inflammatory cytokines (PubMed:28470344).<ref>PMID:12092808</ref> <ref>PMID:17383961</ref> <ref>PMID:19109923</ref> <ref>PMID:21790704</ref> <ref>PMID:27318092</ref> <ref>PMID:28041800</ref> <ref>PMID:28470344</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-The crystal structure of AOL (a fucose-specific lectin of Aspergillus oryzae) has been solved by SAD (single-wavelength anomalous diffraction) and MAD (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. The overall structure is a six-bladed beta-propeller similar to that of other fucose-specific lectins. The fucose moieties of the seleno-fucosides are located in six fucose-binding sites. Although the Arg and Glu/Gln residues bound to the fucose moiety are common to all fucose-binding sites, the amino-acid residues involved in fucose binding at each site are not identical. The varying peak heights of the seleniums in the electron density map suggest that each fucose-binding site has a different carbohydrate binding affinity.
-Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin.,Makyio H, Shimabukuro J, Suzuki T, Imamura A, Ishida H, Kiso M, Ando H, Kato R Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30987-1. doi:, 10.1016/j.bbrc.2016.06.069. PMID:27318092<ref>PMID:27318092</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 5eo7" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

5eo7: Difference between revisions

Latest revision as of 09:11, 20 March 2024

Crystal structure of AOL

Structural highlights

Function

References

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