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==STRUCTURE OF THE MMTV NUCLEOCAPSID PROTEIN (C-TERMINAL ZINC FINGER)==
==STRUCTURE OF THE MMTV NUCLEOCAPSID PROTEIN (C-TERMINAL ZINC FINGER)==
<StructureSection load='1dsv' size='340' side='right'caption='[[1dsv]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1dsv' size='340' side='right'caption='[[1dsv]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1dsv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mmtv Mmtv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DSV FirstGlance]. <br>
<table><tr><td colspan='2'>[[1dsv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mouse_mammary_tumor_virus Mouse mammary tumor virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DSV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dsq|1dsq]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dsv OCA], [https://pdbe.org/1dsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dsv RCSB], [https://www.ebi.ac.uk/pdbsum/1dsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dsv ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dsv OCA], [https://pdbe.org/1dsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dsv RCSB], [https://www.ebi.ac.uk/pdbsum/1dsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dsv ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/GAG_MMTVC GAG_MMTVC]] p10 is the matrix protein.  P14 is the nucleocapsid protein. Binds to single stranded DNA.  p27 is the capsid protein.  
[https://www.uniprot.org/uniprot/GAG_MMTVC GAG_MMTVC] p10 is the matrix protein.  P14 is the nucleocapsid protein. Binds to single stranded DNA.  p27 is the capsid protein.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dsv ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dsv ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The nucleocapsid protein (NC) from the mouse mammary tumor virus (MMTV) has been overexpressed in Escherichia coli and purified to homogeneity for structural studies by nuclear magnetic resonance (NMR) spectroscopy. The protein contains two copies of a conserved zinc-coordinating "CCHC array" or "zinc knuckle" motif common to the nucleocapsid proteins of nearly all known retroviruses. The residues comprising and adjacent to the zinc knuckles were assigned by standard two-dimensional (1)H and three-dimensional (1)H-(15)N NMR methods; the rotational dynamic properties of the protein were determined from (15)N relaxation experiments, and distance restraints derived from the nuclear Overhauser effect (NOE) data were used to calculate the three-dimensional structure. The (1)H-(1)H NOE and (15)N relaxation data indicate that the two zinc knuckles do not interact with each other, but instead behave as independently folded domains connected by a flexible 13-residue linker segment. The proximal zinc knuckle folds in a manner that is essentially identical to that observed previously for the two zinc knuckles of the human immunodeficiency virus type 1 nucleocapsid protein and for the moloney murine leukemia virus nucleocapsid zinc knuckle domain. However, the distal zinc knuckle of MMTV NC exhibits a rare three-dimensional fold that includes an additional C-terminal beta-hairpin. A similar C-terminal reverse turn-like structure was observed recently in the distal zinc knuckle of the Mason-Pfizer monkey virus nucleocapsid protein [Gao, Y., et al. (1998) Protein Sci. 7, 2265-2280]. However, despite a high degree of sequence homology, the conformation and orientation of the beta-hairpin in MMTV NC is significantly different from that of the reverse turn in MPMV NC. The results support the conclusion that structural features of NC zinc knuckle domains can vary significantly among the different genera of retroviridae, and are discussed in terms of the recent and surprising discovery that MMTV NC can facilitate packaging of the HIV-1 genome in chimeric MMTV mutants.
The NMR structure of the nucleocapsid protein from the mouse mammary tumor virus reveals unusual folding of the C-terminal zinc knuckle.,Klein DJ, Johnson PE, Zollars ES, De Guzman RN, Summers MF Biochemistry. 2000 Feb 22;39(7):1604-12. PMID:10677209<ref>PMID:10677209</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1dsv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mmtv]]
[[Category: Mouse mammary tumor virus]]
[[Category: Guzman, R N.De]]
[[Category: De Guzman RN]]
[[Category: Johnson, P E]]
[[Category: Johnson PE]]
[[Category: Klein, D J]]
[[Category: Klein DJ]]
[[Category: Summers, M F]]
[[Category: Summers MF]]
[[Category: Zollars, E S]]
[[Category: Zollars ES]]
[[Category: Cchc type zinc finger]]
[[Category: Viral protein]]
[[Category: Virus/viral protein]]

Revision as of 09:53, 20 March 2024

STRUCTURE OF THE MMTV NUCLEOCAPSID PROTEIN (C-TERMINAL ZINC FINGER)

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