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==Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone==
==Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone==
<StructureSection load='5xxm' size='340' side='right' caption='[[5xxm]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='5xxm' size='340' side='right'caption='[[5xxm]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5xxm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactn Bactn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XXM FirstGlance]. <br>
<table><tr><td colspan='2'>[[5xxm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XXM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGC:(3S,4R,5R,6S)-3,4,5-TRIHYDROXY-6-(HYDROXYMETHYL)TETRAHYDRO-2H-PYRAN-2-ONE'>LGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BT_3567 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226186 BACTN])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGC:(3S,4R,5R,6S)-3,4,5-TRIHYDROXY-6-(HYDROXYMETHYL)TETRAHYDRO-2H-PYRAN-2-ONE'>LGC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxm OCA], [http://pdbe.org/5xxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xxm RCSB], [http://www.ebi.ac.uk/pdbsum/5xxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxm ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xxm OCA], [https://pdbe.org/5xxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xxm RCSB], [https://www.ebi.ac.uk/pdbsum/5xxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xxm ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8A1U1_BACTN Q8A1U1_BACTN]
BT_3567 protein, a putative beta-glucosidase from Bacteroides thetaiotaomicron, exhibits higher activity toward Sop3-5 (Sopn , n: degree of polymerization of beta-1,2-glucooligosaccharides) than toward Sop2 , unlike a known beta-glucosidase from Listeria innocua which predominantly prefers Sop2 . In the complex structure determined by soaking of a D286N mutant crystal with Sop4 , a Sop3 moiety was observed at subsites -1 to +2. The glucose moiety at subsite +2 forms a hydrogen bond with Asn81, which is replaced with Gly in the L. innocua beta-glucosidase. The Km values of the N81G mutant for Sop3-5 are much higher than those of the wild-type, suggesting that Asn81 contributes to the binding to substrates longer than Sop3 .


Function and structure relationships of a beta-1,2-glucooligosaccharide-degrading beta-glucosidase.,Ishiguro R, Tanaka N, Abe K, Nakajima M, Maeda T, Miyanaga A, Takahashi Y, Sugimoto N, Nakai H, Taguchi H FEBS Lett. 2017 Dec;591(23):3926-3936. doi: 10.1002/1873-3468.12911. Epub 2017, Nov 24. PMID:29131329<ref>PMID:29131329</ref>
==See Also==
 
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xxm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bactn]]
[[Category: Bacteroides thetaiotaomicron VPI-5482]]
[[Category: Abe, K]]
[[Category: Large Structures]]
[[Category: Ishiguro, R]]
[[Category: Abe K]]
[[Category: Maeda, T]]
[[Category: Ishiguro R]]
[[Category: Miyanaga, A]]
[[Category: Maeda T]]
[[Category: Nakai, H]]
[[Category: Miyanaga A]]
[[Category: Nakajima, M]]
[[Category: Nakai H]]
[[Category: Sugimoto, N]]
[[Category: Nakajima M]]
[[Category: Taguchi, H]]
[[Category: Sugimoto N]]
[[Category: Takahash, Y]]
[[Category: Taguchi H]]
[[Category: Tanaka, N]]
[[Category: Takahash Y]]
[[Category: Beta-glucosidase]]
[[Category: Tanaka N]]
[[Category: Glycoside hydrolase family 3]]
[[Category: Hydrolase]]
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