1rbj: Difference between revisions
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'''RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))''' | '''RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))''' | ||
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[[Category: McPherson, A.]] | [[Category: McPherson, A.]] | ||
[[Category: Williams, R.]] | [[Category: Williams, R.]] | ||
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Revision as of 04:18, 3 May 2008
RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))
Overview
The structure of a tetragonal crystal of bovine pancreatic RNase B complexed with d(pA)(4) was determined by molecular replacement and difference Fourier methods. This crystal belongs to space group P4(1)2(1)2 and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include any polysaccharide residues or solvent molecules. After refinement at 2.7 A, the R value was 0.163 with acceptable stereochemistry. The model illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.
About this Structure
1RBJ is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure of a ribonuclease B+d(pA)4 complex., Ko TP, Williams R, McPherson A, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):160-4. PMID:15299737 Page seeded by OCA on Sat May 3 07:18:25 2008
