5zey: Difference between revisions

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<SX load='5zey' size='340' side='right' viewer='molstar' caption='[[5zey]], [[Resolution|resolution]] 12.50&Aring;' scene=''>
<SX load='5zey' size='340' side='right' viewer='molstar' caption='[[5zey]], [[Resolution|resolution]] 12.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5zey]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli], [https://en.wikipedia.org/wiki/Mycs2 Mycs2] and [https://en.wikipedia.org/wiki/Mycobacterium_smegmatis_(strain_atcc_700084_/_mc(2)155) Mycobacterium smegmatis (strain atcc 700084 / mc(2)155)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZEY FirstGlance]. <br>
<table><tr><td colspan='2'>[[5zey]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZEY FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zey OCA], [https://pdbe.org/5zey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zey RCSB], [https://www.ebi.ac.uk/pdbsum/5zey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zey ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zey OCA], [https://pdbe.org/5zey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zey RCSB], [https://www.ebi.ac.uk/pdbsum/5zey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zey ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/SSRP_MYCS2 SSRP_MYCS2]
Ribosomes are the dynamic protein synthesis machineries of the cell. They may exist in different functional states in the cell. Therefore, it is essential to have structural information on these different functional states of ribosomes to understand their mechanism of action. Here, we present single particle cryo-EM reconstructions of the Mycobacterium smegmatis 70S ribosomes in the hibernating state (with HPF), trans-translating state (with tmRNA), and the P/P state (with P-tRNA) resolved to 4.1, 12.5, and 3.4 A, respectively. A comparison of the P/P state with the hibernating state provides possible functional insights about the Mycobacteria-specific helix H54a rRNA segment. Interestingly, densities for all the four OB domains of bS1 protein is visible in the hibernating 70S ribosome displaying the molecular details of bS1-70S interactions. Our structural data shows a Mycobacteria-specific H54a-bS1 interaction which seems to prevent subunit dissociation and degradation during hibernation without the formation of 100S dimer. This indicates a new role of bS1 protein in 70S protection during hibernation in Mycobacteria in addition to its conserved function during translation initiation.
 
Structures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNA.,Mishra S, Ahmed T, Tyagi A, Shi J, Bhushan S Sci Rep. 2018 Sep 11;8(1):13587. doi: 10.1038/s41598-018-31850-3. PMID:30206241<ref>PMID:30206241</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5zey" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mycs2]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Ahmed, T]]
[[Category: Ahmed T]]
[[Category: Bhushan, S]]
[[Category: Bhushan S]]
[[Category: Mishra, S]]
[[Category: Mishra S]]
[[Category: Shi, J]]
[[Category: Shi J]]
[[Category: Tyagi, A]]
[[Category: Tyagi A]]
[[Category: Rna binding protein-rna complex]]
[[Category: Trans-translating state]]

Latest revision as of 10:27, 27 March 2024

M. smegmatis Trans-translation state 70S ribosome

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