6j6j: Difference between revisions

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<SX load='6j6j' size='340' side='right' viewer='molstar' caption='[[6j6j]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<SX load='6j6j' size='340' side='right' viewer='molstar' caption='[[6j6j]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6j6j]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6J6J FirstGlance]. <br>
<table><tr><td colspan='2'>[[6j6j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J6J FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6j6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6j OCA], [http://pdbe.org/6j6j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j6j RCSB], [http://www.ebi.ac.uk/pdbsum/6j6j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6j ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j6j OCA], [https://pdbe.org/6j6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j6j RCSB], [https://www.ebi.ac.uk/pdbsum/6j6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j6j ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 A resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.


Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.,Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW Nat Commun. 2019 Jun 3;10(1):2386. doi: 10.1038/s41467-019-10368-w. PMID:31160591<ref>PMID:31160591</ref>
==See Also==
 
*[[Avidin 3D structures|Avidin 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6j6j" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
[[Category: Fan, X]]
[[Category: Fan X]]
[[Category: Lei, J L]]
[[Category: Lei JL]]
[[Category: Wang, H W]]
[[Category: Wang HW]]
[[Category: Wang, J]]
[[Category: Wang J]]
[[Category: Cytosolic protein]]
[[Category: Streptavidin]]

Revision as of 10:37, 27 March 2024

Biotin-bound streptavidin

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