6lcm: Difference between revisions

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<StructureSection load='6lcm' size='340' side='right'caption='[[6lcm]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='6lcm' size='340' side='right'caption='[[6lcm]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6lcm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LCM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LCM FirstGlance]. <br>
<table><tr><td colspan='2'>[[6lcm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LCM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I3C:5-AMINO-2,4,6-TRIIODOBENZENE-1,3-DICARBOXYLIC+ACID'>I3C</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lcm OCA], [http://pdbe.org/6lcm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lcm RCSB], [http://www.ebi.ac.uk/pdbsum/6lcm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lcm ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=I3C:5-AMINO-2,4,6-TRIIODOBENZENE-1,3-DICARBOXYLIC+ACID'>I3C</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lcm OCA], [https://pdbe.org/6lcm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lcm RCSB], [https://www.ebi.ac.uk/pdbsum/6lcm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lcm ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/B4FCI7_MAIZE B4FCI7_MAIZE]
Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. HJ resolvases have been identified across all kingdoms of life, members of which exhibit sequence-dependent HJ resolution. However, the molecular basis of sequence selectivity remains largely unknown. Here, we present the chloroplast resolvase MOC1, which cleaves HJ in a cytosine-dependent manner. We determine the crystal structure of MOC1 with and without HJs. MOC1 exhibits an RNase H fold, belonging to the retroviral integrase family. MOC1 functions as a dimer, and the HJ is embedded into the basic cleft of the dimeric enzyme. We characterize a base recognition loop (BR loop) that protrudes into and opens the junction. Residues from the BR loop intercalate into the bases, disrupt the C-G base pairing at the crossover and recognize the cytosine, providing the molecular basis for sequence-dependent HJ resolution by a resolvase.


Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1.,Yan J, Hong S, Guan Z, He W, Zhang D, Yin P Nat Commun. 2020 Mar 17;11(1):1417. doi: 10.1038/s41467-020-15242-8. PMID:32184398<ref>PMID:32184398</ref>
==See Also==
 
*[[Resolvase 3D structures|Resolvase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lcm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Guan, Z Y]]
[[Category: Zea mays]]
[[Category: Hong, S X]]
[[Category: Guan ZY]]
[[Category: Yan, J J]]
[[Category: Hong SX]]
[[Category: Yin, P]]
[[Category: Yan JJ]]
[[Category: Chloroplast]]
[[Category: Yin P]]
[[Category: Holliday junction]]
[[Category: Hydrolase]]
[[Category: Plant protein]]
[[Category: Resolvase]]

Latest revision as of 10:46, 27 March 2024

Crystal structure of chloroplast resolvase ZmMOC1 with the magic triangle I3C

6lcm, resolution 2.50Å

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