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==XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE==
==XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE==
<StructureSection load='1fre' size='340' side='right'caption='[[1fre]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='1fre' size='340' side='right'caption='[[1fre]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1fre]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FRE FirstGlance]. <br>
<table><tr><td colspan='2'>[[1fre]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FRE FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fre OCA], [https://pdbe.org/1fre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fre RCSB], [https://www.ebi.ac.uk/pdbsum/1fre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fre ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fre OCA], [https://pdbe.org/1fre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fre RCSB], [https://www.ebi.ac.uk/pdbsum/1fre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fre ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/NF7B_XENLA NF7B_XENLA]] Transcription factor that determines dorsal-ventral body axis.<ref>PMID:1936552</ref>
[https://www.uniprot.org/uniprot/NF7B_XENLA NF7B_XENLA] Transcription factor that determines dorsal-ventral body axis.<ref>PMID:1936552</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fre ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fre ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Xenopus nuclear factor XNF7, a maternally expressed protein, functions in patterning of the embryo. XNF7 contains a number of defined protein domains implicated in the regulation of some developmental processes. Among these is a tripartite motif comprising a zinc-binding RING finger and B-box domain next to a predicted alpha-helical coiled-coil domain. Interestingly, this motif is found in a variety of protein including several proto-oncoproteins. Here we describe the solution structure of the XNF7 B-box zinc-binding domain determined at physiological pH by 1H NMR methods. The B-box structure represents the first three-dimensional structure of this new motif and comprises a monomer have two beta-strands, two helical turns and three extended loop regions packed in a novel topology. The r.m.s. deviation for the best 18 structures is 1.15 A for backbone atoms and 1.94 A for all atoms. Structure calculations and biochemical data shows one zinc atom ligated in a Cys2-His2 tetrahedral arrangement. We have used mutant peptides to determine the metal ligation scheme which surprisingly shows that not all of the seven conserved cysteines/histidines in the B-box motif are involved in metal ligation. The B-box structure is not similar in tertiary fold to any other known zinc-binding motif.
Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development.,Borden KL, Lally JM, Martin SR, O'Reilly NJ, Etkin LD, Freemont PS EMBO J. 1995 Dec 1;14(23):5947-56. PMID:8846787<ref>PMID:8846787</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1fre" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: African clawed frog]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Borden, K L.B]]
[[Category: Xenopus laevis]]
[[Category: Freemont, P S]]
[[Category: Borden KLB]]
[[Category: Bbox]]
[[Category: Freemont PS]]
[[Category: Development]]
[[Category: Mid-blastula-transition]]
[[Category: Xnf7]]
[[Category: Zinc-binding protein]]
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