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==NMR Solution Structure of Pisum sativum defensin 1 (Psd1)==
==NMR Solution Structure of Pisum sativum defensin 1 (Psd1)==
<StructureSection load='1jkz' size='340' side='right'caption='[[1jkz]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1jkz' size='340' side='right'caption='[[1jkz]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1jkz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JKZ FirstGlance]. <br>
<table><tr><td colspan='2'>[[1jkz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JKZ FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jkz OCA], [https://pdbe.org/1jkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jkz RCSB], [https://www.ebi.ac.uk/pdbsum/1jkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jkz ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jkz OCA], [https://pdbe.org/1jkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jkz RCSB], [https://www.ebi.ac.uk/pdbsum/1jkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jkz ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEF1_PEA DEF1_PEA] Possesses antifungal activity sensitive to inorganic cations.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jkz ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jkz ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pisum sativum defensin 1 (Psd1) is a 46 amino acid residue plant defensin isolated from seeds of pea. The three-dimensional structure in solution of Psd1 was determined by two-dimensional NMR data recorded at 600 MHz. Experimental restraints were used for structure calculation using CNS and torsion-angle molecular dynamics. The 20 lowest energy structures were selected and further subjected to minimization, giving a root-mean-square deviation of 0.78(+/- 0.22) A in the backbone and 1.91(+/-0.60) A for over all atoms of the molecule. The protein has a globular fold with a triple-stranded antiparalell beta-sheet and an alpha-helix (from residue Asn17 to Leu27). Psd1 presents the so called "cysteine stabilized alpha/beta motif" and presents identical three-dimensional topology in the backbone with other defensins and neurotoxins. Comparison of the electrostatic surface potential among proteins with high three-dimensional (selected using the softwares TOP and DALI) topology gave insights into the mode of action of Psd1. The surface topologies between proteins that present antifungal activity or sodium channel inhibiting activity are different. On the other hand the surface topology presents several common features with potassium channel inhibitors, suggesting that Psd1 presents this activity. Other common features with potassium channel inhibitors were found including the presence of a lysine residue essential for inhibitory activity. The identity of Psd1 in primary sequence is not enough to infer a mechanism of action, in contrast with the strategy proposed here.
Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action.,Almeida MS, Cabral KM, Kurtenbach E, Almeida FC, Valente AP J Mol Biol. 2002 Jan 25;315(4):749-57. PMID:11812144<ref>PMID:11812144</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1jkz" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Plant defensin|Plant defensin]]
*[[Plant defensin|Plant defensin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
[[Category: Almeida, F C.L]]
[[Category: Almeida FCL]]
[[Category: Almeida, M S]]
[[Category: Almeida MS]]
[[Category: Cabral, K M.S]]
[[Category: Cabral KMS]]
[[Category: Kurtenbach, E]]
[[Category: Kurtenbach E]]
[[Category: Valente, A P]]
[[Category: Valente AP]]
[[Category: Antifungal]]
[[Category: Antifungal protein]]
[[Category: Cys-rich]]
[[Category: Plant defensin]]

Revision as of 07:52, 3 April 2024

NMR Solution Structure of Pisum sativum defensin 1 (Psd1)

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