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<StructureSection load='1kxi' size='340' side='right'caption='[[1kxi]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
<StructureSection load='1kxi' size='340' side='right'caption='[[1kxi]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1kxi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chinese_cobra Chinese cobra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KXI FirstGlance]. <br>
<table><tr><td colspan='2'>[[1kxi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KXI FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kxi OCA], [https://pdbe.org/1kxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kxi RCSB], [https://www.ebi.ac.uk/pdbsum/1kxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kxi ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kxi OCA], [https://pdbe.org/1kxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kxi RCSB], [https://www.ebi.ac.uk/pdbsum/1kxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kxi ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/3SOF5_NAJAT 3SOF5_NAJAT]] Non-cytotoxic protein that does not show lytic and hemolytic activities, but can induce aggregation and fusion of sphingomyelin vesicles (PubMed:8182052). It binds to integrin alpha-V/beta-3 (ITGAV/ITGB3) with high affinity, and it inhibits osteoclast differentiation and bone resorption in mice, probably due to binding to integrin alpha-V/beta-3 (PubMed:16407244).<ref>PMID:14743531</ref> <ref>PMID:16407244</ref> <ref>PMID:8182052</ref> <ref>PMID:8703922</ref>
[https://www.uniprot.org/uniprot/3SOF5_NAJAT 3SOF5_NAJAT] Non-cytotoxic protein that does not show lytic and hemolytic activities, but can induce aggregation and fusion of sphingomyelin vesicles (PubMed:8182052). It binds to integrin alpha-V/beta-3 (ITGAV/ITGB3) with high affinity, and it inhibits osteoclast differentiation and bone resorption in mice, probably due to binding to integrin alpha-V/beta-3 (PubMed:16407244).<ref>PMID:14743531</ref> <ref>PMID:16407244</ref> <ref>PMID:8182052</ref> <ref>PMID:8703922</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kxi ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kxi ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of cardiotoxin V from Taiwan cobra venom (CTX A5) has been solved at pH 8.5 and refined to an R-factor of 20.7% for 7013 reflections [&gt;2sigma(F)] between 8- and 2.19-A resolution. The refined model shows that CTX A5 exists as a dimer. The assembly consists of 974 non-hydrogen atoms from 124 residues and 73 water molecules. The global monomeric structure is similar to that determined by NMR at pH 3.7, characterized by a core formed by two beta-sheets connected with three-finger loops. However, local conformational differences are detected in two functionally important regions, loops I and II. A disparity between the NMR and X-ray structure of CTX A5 is detected near the tip of loop I and can be attributed to the difference in the protonation state of His4 at different pH, resulting in a reorientation of the His4 imidazole ring. A concerted motion of amino acid side chains located near His4 is detected and possibly contributes to the pH-dependent binding ability of CTX A5 to phospholipid model membranes. The second difference, detected at the tip of loop II, is due to the hydrophobic contact between CTX dimers in the crystal packing and the interaction of water molecules with amino acid residues in the loop II region of the CTX containing Pro31 (P-type CTX). This interaction forces loop II into a more rigid omega shape bridging the main chain at positions 27 and 34, contradictory to the flexible, tapering shape detected by NMR. Thus, a novel continuous hydrophobic column capable of binding to and possibly penetrating the membrane lipid bilayer is formed by the tips of the three-finger loops. In this respect, the X-ray crystal structure of CTX A5 may represent the CTX structure in the membrane-binding mode.
Crystal structure of cardiotoxin V from Taiwan cobra venom: pH-dependent conformational change and a novel membrane-binding motif identified in the three-finger loops of P-type cardiotoxin.,Sun YJ, Wu WG, Chiang CM, Hsin AY, Hsiao CD Biochemistry. 1997 Mar 4;36(9):2403-13. PMID:9054545<ref>PMID:9054545</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1kxi" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chinese cobra]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chiang, C M]]
[[Category: Naja atra]]
[[Category: Hsiao, C D]]
[[Category: Chiang C-M]]
[[Category: Hsin, A Y]]
[[Category: Hsiao C-D]]
[[Category: Sun, Y J]]
[[Category: Hsin A-Y]]
[[Category: Wu, W G]]
[[Category: Sun Y-J]]
[[Category: Cardiotoxin]]
[[Category: Wu W-G]]
[[Category: Cytotoxin]]
[[Category: Multigene family]]
[[Category: Venom]]

Revision as of 08:04, 3 April 2024

STRUCTURE OF CYTOTOXIN HOMOLOG PRECURSOR

1kxi, resolution 2.19Å

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