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==Solution Structure of Novel Disintegrin Salmosin==
==Solution Structure of Novel Disintegrin Salmosin==
<StructureSection load='1l3x' size='340' side='right'caption='[[1l3x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1l3x' size='340' side='right'caption='[[1l3x]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1l3x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloydius_blomhoffi_brevicaudus Gloydius blomhoffi brevicaudus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1iq2 1iq2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L3X FirstGlance]. <br>
<table><tr><td colspan='2'>[[1l3x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloydius_brevicaudus Gloydius brevicaudus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1iq2 1iq2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L3X FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1laz|1laz]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3x OCA], [https://pdbe.org/1l3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l3x RCSB], [https://www.ebi.ac.uk/pdbsum/1l3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3x ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l3x OCA], [https://pdbe.org/1l3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l3x RCSB], [https://www.ebi.ac.uk/pdbsum/1l3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l3x ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/VM2HS_GLOBR VM2HS_GLOBR]] Metalloproteinase hxl-1: snake venom metalloproteinase that impairs hemostasis in the envenomed animal (By similarity).<ref>PMID:9722022</ref> <ref>PMID:9856345</ref> <ref>PMID:10446992</ref> <ref>PMID:10944460</ref> <ref>PMID:14977354</ref>  Disintegrin salmosin-1: inhibits GPIIb/GPIIIa (ITGA2B/ITGB3) binding to immobilized fibrinogen with an IC(50) of 2.2 nM and ADP-induced platelet aggregation with an IC(50) of 131 nM, respectively. Inhibits angiogenesis.<ref>PMID:9722022</ref> <ref>PMID:9856345</ref> <ref>PMID:10446992</ref> <ref>PMID:10944460</ref> <ref>PMID:14977354</ref>
[https://www.uniprot.org/uniprot/VM2HS_GLOBR VM2HS_GLOBR] Metalloproteinase hxl-1: snake venom metalloproteinase that impairs hemostasis in the envenomed animal (By similarity).<ref>PMID:9722022</ref> <ref>PMID:9856345</ref> <ref>PMID:10446992</ref> <ref>PMID:10944460</ref> <ref>PMID:14977354</ref>  Disintegrin salmosin-1: inhibits GPIIb/GPIIIa (ITGA2B/ITGB3) binding to immobilized fibrinogen with an IC(50) of 2.2 nM and ADP-induced platelet aggregation with an IC(50) of 131 nM, respectively. Inhibits angiogenesis.<ref>PMID:9722022</ref> <ref>PMID:9856345</ref> <ref>PMID:10446992</ref> <ref>PMID:10944460</ref> <ref>PMID:14977354</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l3x ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l3x ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Disintegrins are potent inhibitors of both platelet aggregation and integrin-dependent cell adhesion. A new disintegrin, salmosin, isolated from the venom of the Korean snake Agkistrodon halys brevicaudus, has been characterized by mass spectrometry and NMR spectroscopy, and its in vitro biological activity has been assessed. The IC(50) value of the purified salmosin was determined to be 2.2 nM in an assay for the inhibition of glycoprotein IIb-IIIa/fibrinogen interaction. Salmosin also inhibited the bovine capillary endothelial cell proliferation induced by bFGF in a dose-dependent manner. The NMR solution structures were well converged with a root-mean-square deviation of 0.76 A for backbone atoms among the 20 lowest energy structures, except for the arginylglycylaspartic acid (RGD) loop. The structure revealed that the conserved RGD motif with an unusual finger shape is distal from the rigid core of the C-terminal domain. Furthermore, even though the RGD motif did not interact with the hydrophobic core of the protein, it was stabilized by a network of molecular contacts through a small antiparallel beta-sheet comprising residues of Ile46-Ala50 and Asp54-Tyr58. Last, the electrostatic charge distribution on the surface of salmosin differs dramatically from that of other disintegrin proteins in that there is a cluster of negatively charged residues in close proximity to the RGD loop.
Solution structure of a novel disintegrin, salmosin, from Agkistrondon halys venom.,Shin J, Hong SY, Chung K, Kang I, Jang Y, Kim DS, Lee W Biochemistry. 2003 Dec 16;42(49):14408-15. PMID:14661951<ref>PMID:14661951</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1l3x" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Gloydius blomhoffi brevicaudus]]
[[Category: Gloydius brevicaudus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lee, W]]
[[Category: Lee W]]
[[Category: Shin, J]]
[[Category: Shin J]]
[[Category: Disintegrin]]
[[Category: Protein binding]]
[[Category: Rgd]]
[[Category: Snake venome]]
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