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==Solution structure of the PWI motif from SRm160==
==Solution structure of the PWI motif from SRm160==
<StructureSection load='1mp1' size='340' side='right'caption='[[1mp1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1mp1' size='340' side='right'caption='[[1mp1]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1mp1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MP1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1mp1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MP1 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SRm160 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp1 OCA], [https://pdbe.org/1mp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mp1 RCSB], [https://www.ebi.ac.uk/pdbsum/1mp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mp1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mp1 OCA], [https://pdbe.org/1mp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mp1 RCSB], [https://www.ebi.ac.uk/pdbsum/1mp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mp1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/SRRM1_HUMAN SRRM1_HUMAN]] Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.<ref>PMID:9531537</ref> <ref>PMID:10339552</ref> <ref>PMID:10668804</ref> <ref>PMID:11739730</ref> <ref>PMID:12944400</ref> <ref>PMID:12600940</ref>
[https://www.uniprot.org/uniprot/SRRM1_HUMAN SRRM1_HUMAN] Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.<ref>PMID:9531537</ref> <ref>PMID:10339552</ref> <ref>PMID:10668804</ref> <ref>PMID:11739730</ref> <ref>PMID:12944400</ref> <ref>PMID:12600940</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mp1 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mp1 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.
Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.,Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ Genes Dev. 2003 Feb 15;17(4):461-75. PMID:12600940<ref>PMID:12600940</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1mp1" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Arrowsmith, C H]]
[[Category: Arrowsmith CH]]
[[Category: Blencowe, B J]]
[[Category: Blencowe BJ]]
[[Category: Bowman, J]]
[[Category: Bowman J]]
[[Category: Cox, B]]
[[Category: Cox B]]
[[Category: Graveley, B R]]
[[Category: Graveley BR]]
[[Category: Lambermon, M]]
[[Category: Lambermon M]]
[[Category: Lu, Y]]
[[Category: Lu Y]]
[[Category: McCracken, S]]
[[Category: McCracken S]]
[[Category: Pineda-Lucena, A]]
[[Category: Pineda-Lucena A]]
[[Category: Szymczyna, B R]]
[[Category: Szymczyna BR]]
[[Category: Four helix bundle]]
[[Category: Rna binding protein]]

Revision as of 08:38, 10 April 2024

Solution structure of the PWI motif from SRm160

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