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==Sensory domain of the membraneous two-component fumarate sensor DcuS of E. coli==
==Sensory domain of the membraneous two-component fumarate sensor DcuS of E. coli==
<StructureSection load='1ojg' size='340' side='right'caption='[[1ojg]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='1ojg' size='340' side='right'caption='[[1ojg]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1ojg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OJG FirstGlance]. <br>
<table><tr><td colspan='2'>[[1ojg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OJG FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojg OCA], [https://pdbe.org/1ojg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ojg RCSB], [https://www.ebi.ac.uk/pdbsum/1ojg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojg ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ojg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ojg OCA], [https://pdbe.org/1ojg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ojg RCSB], [https://www.ebi.ac.uk/pdbsum/1ojg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ojg ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/DCUS_ECOLI DCUS_ECOLI]] Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation.  
[https://www.uniprot.org/uniprot/DCUS_ECOLI DCUS_ECOLI] Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ojg ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ojg ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the water-soluble, periplasmic domain of the fumarate sensor DcuS (DcuS-pd) has been determined by NMR spectroscopy in solution. DcuS is a prototype for a sensory histidine kinase with transmembrane signal transfer. DcuS belongs to the CitA family of sensors that are specific for sensing di- and tricarboxylates. The periplasmic domain is folded autonomously and shows helices at the N and the C terminus, suggesting direct linking or connection to helices in the two transmembrane regions. The structure constitutes a novel fold. The nearest structural neighbor is the Per-Arnt-Sim domain of the photoactive yellow protein that binds small molecules covalently. Residues Arg107, His110, and Arg147 are essential for fumarate sensing and are found clustered together. The structure constitutes the first periplasmic domain of a two component sensory system and is distinctly different from the aspartate sensory domain of the Tar chemotaxis sensor.
The NMR structure of the sensory domain of the membranous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli.,Pappalardo L, Janausch IG, Vijayan V, Zientz E, Junker J, Peti W, Zweckstetter M, Unden G, Griesinger C J Biol Chem. 2003 Oct 3;278(40):39185-8. Epub 2003 Aug 7. PMID:12907689<ref>PMID:12907689</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ojg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Griesinger, C]]
[[Category: Griesinger C]]
[[Category: Janausch, I G]]
[[Category: Janausch IG]]
[[Category: Junker, J]]
[[Category: Junker J]]
[[Category: Pappalardo, L]]
[[Category: Pappalardo L]]
[[Category: Peti, W]]
[[Category: Peti W]]
[[Category: Unden, G]]
[[Category: Unden G]]
[[Category: Vijayan, V]]
[[Category: Vijayan V]]
[[Category: Zientz, E]]
[[Category: Zientz E]]
[[Category: Zweckstetter, M]]
[[Category: Zweckstetter M]]
[[Category: Dcus]]
[[Category: Fumarate]]
[[Category: Histidine kinase]]
[[Category: Transferase]]
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