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<StructureSection load='1or0' size='340' side='right'caption='[[1or0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='1or0' size='340' side='right'caption='[[1or0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1or0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseu7 Pseu7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OR0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1or0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._SY-77-1 Pseudomonas sp. SY-77-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OR0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1oqz|1oqz]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1or0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1or0 OCA], [https://pdbe.org/1or0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1or0 RCSB], [https://www.ebi.ac.uk/pdbsum/1or0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1or0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1or0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1or0 OCA], [https://pdbe.org/1or0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1or0 RCSB], [https://www.ebi.ac.uk/pdbsum/1or0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1or0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/G7AC_PSEU7 G7AC_PSEU7]] Catalyzes the deacylation of 7 beta-(4-carboxybutanamido)cephalosporanic acid (glutaryl-7-aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA).<ref>PMID:2993240</ref>
[https://www.uniprot.org/uniprot/G7AC_PSEU7 G7AC_PSEU7] Catalyzes the deacylation of 7 beta-(4-carboxybutanamido)cephalosporanic acid (glutaryl-7-aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA).<ref>PMID:2993240</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1or0 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1or0 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glutaryl 7-aminocephalosporanic acid acylase (GCA, EC 3.5.1.11) is a member of N-terminal nucleophile (Ntn) hydrolases. The native enzyme is an (alpha beta)(2) heterotetramer originated from an enzymatically inactive precursor of a single polypeptide. The activation of precursor GCA consists of primary and secondary autoproteolytic cleavages, generating a terminal residue with both a nucleophile and a base and releasing a nine amino acid spacer peptide. We have determined the crystal structures of the recombinant selenomethionyl native and S170A mutant precursor from Pseudomonas sp. strain GK16. Precursor activation is likely triggered by conformational constraints within the spacer peptide, probably inducing a peptide flip. Autoproteolytic site solvent molecules, which have been trapped in a hydrophobic environment by the spacer peptide, may play a role as a general base for nucleophilic attack. The activation results in building up a catalytic triad composed of Ser170/His192/Glu624. However, the triad is not linked to the usual hydroxyl but the free alpha-amino group of the N-terminal serine residue of the native GCA. Mutagenesis and structural data support the notion that the stabilization of a transient hydroxazolidine ring during autoproteolysis would be critical during the N --&gt; O acyl shift. The autoproteolytic activation mechanism for GCA is described.
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.,Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH Biochemistry. 2003 Apr 15;42(14):4084-93. PMID:12680762<ref>PMID:12680762</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1or0" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Pseu7]]
[[Category: Pseudomonas sp. SY-77-1]]
[[Category: Dauter, Z]]
[[Category: Dauter Z]]
[[Category: Kim, J K]]
[[Category: Kim JK]]
[[Category: Kim, K H]]
[[Category: Kim KH]]
[[Category: Lee, Y S]]
[[Category: Lee YS]]
[[Category: Park, S S]]
[[Category: Park SS]]
[[Category: Rhee, S]]
[[Category: Rhee S]]
[[Category: Yang, I S]]
[[Category: Yang IS]]
[[Category: Glutaryl 7-aminocephalosporanic acid]]
[[Category: Glutaryl 7-aminocephalosporanic acid acylase]]
[[Category: Hydrolase]]

Revision as of 05:49, 17 April 2024

Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

1or0, resolution 2.00Å

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