1pfd: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:


==THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES==
==THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES==
<StructureSection load='1pfd' size='340' side='right'caption='[[1pfd]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''>
<StructureSection load='1pfd' size='340' side='right'caption='[[1pfd]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1pfd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFD FirstGlance]. <br>
<table><tr><td colspan='2'>[[1pfd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Petroselinum_crispum Petroselinum crispum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PFD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ferredoxin--nitrate_reductase Ferredoxin--nitrate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.7.2 1.7.7.2] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfd OCA], [https://pdbe.org/1pfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfd RCSB], [https://www.ebi.ac.uk/pdbsum/1pfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfd ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pfd OCA], [https://pdbe.org/1pfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pfd RCSB], [https://www.ebi.ac.uk/pdbsum/1pfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pfd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7M1S1_PETCR Q7M1S1_PETCR]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 18: Line 20:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pfd ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pfd ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The [2Fe-2S]ferredoxin I (Fd I) from parsley leaves (Mr = 10,500; 96 amino acids) in the Fe(III)-Fe(III) oxidized form has been studied by 1H-NMR spectroscopy. Sequence-specific 1H-NMR assignments were obtained through two-dimensional classical double-quantum-filtered-COSY, NOESY and TOCSY spectra. NOEs between protons as close as 5.6 A from the paramagnetic Fe(III) atoms were observed at 800 MHz. A total of 3066 NOEs (of which 2533 are meaningful) and 18 distance constraints taken from X-ray crystallography of the Fe2S2 active site were used to obtain the solution structure. From inversion recovery NOESY experiments, 33 longitudinal relaxation rate (Qpara) constraints were used for the structural refinement. The final structure was obtained by a process of restrained energy minimization. Root-mean-square (rmsd) deviation values obtained for the family of 18 structures (with reference to the average structure) are 0.52 +/- 0.10 A and 0.91 +/- 0.12 A for backbone and all heavy atoms respectively. The structure consists of seven-strands of beta-sheets and four short alpha-helices. The quality of the present solution structure is among the best of those reported for [2Fe-2S]ferredoxins. The secondary structure and overall folding are compared with those of Anabaena variabilis Fd and the higher plant Equistum arvense (horse tail) protein determined through X-ray crystallography. The groups believed to be responsible for electron transfer have been analysed.
The solution structure of parsley [2Fe-2S]ferredoxin.,Im SC, Liu G, Luchinat C, Sykes AG, Bertini I Eur J Biochem. 1998 Dec 1;258(2):465-77. PMID:9874213<ref>PMID:9874213</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1pfd" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ferredoxin--nitrate reductase]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Petroselinum crispum]]
[[Category: Petroselinum crispum]]
[[Category: Bertini, I]]
[[Category: Bertini I]]
[[Category: Im, S C]]
[[Category: Im S-C]]
[[Category: Liu, G]]
[[Category: Liu G]]
[[Category: Luchinat, C]]
[[Category: Luchinat C]]
[[Category: Sykes, A G]]
[[Category: Sykes AG]]
[[Category: Electron transport]]
[[Category: Nuclear relaxation]]
[[Category: Paramagnetism]]
[[Category: Solution structure]]

Revision as of 05:55, 17 April 2024

THE SOLUTION STRUCTURE OF HIGH PLANT PARSLEY [2FE-2S] FERREDOXIN, NMR, 18 STRUCTURES

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1pfd&oldid=4129294"