Sandbox 323: Difference between revisions
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Jennah Hoke (talk | contribs) No edit summary |
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The molecular weight of 3DS8 is proposed to be about 28 kDa. | The molecular weight of 3DS8 is proposed to be about 28 kDa. | ||
In the laboratory, the gene was transformed into E. coli bacteria, expressed, and isolated by cell lysis and nickel column. The cell lysis buffer used was a sodium phosphate buffer. From the column, 10 elution fractions were collected. A Bradford assay with standards of 0.125, 0.25, 0.5, 0.75, and 1 mg/mL of Bovine Serum Albumin solution. | |||
==Discussion== | ==Discussion== | ||