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==Solution Structure of Der f 13, Group 13 Allergen from House Dust Mites==
==Solution Structure of Der f 13, Group 13 Allergen from House Dust Mites==
<StructureSection load='2a0a' size='340' side='right'caption='[[2a0a]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2a0a' size='340' side='right'caption='[[2a0a]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2a0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/American_house_dust_mite American house dust mite]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A0A FirstGlance]. <br>
<table><tr><td colspan='2'>[[2a0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dermatophagoides_farinae Dermatophagoides farinae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A0A FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a0a OCA], [https://pdbe.org/2a0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a0a RCSB], [https://www.ebi.ac.uk/pdbsum/2a0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a0a ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a0a OCA], [https://pdbe.org/2a0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a0a RCSB], [https://www.ebi.ac.uk/pdbsum/2a0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a0a ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q1M2P5_DERFA Q1M2P5_DERFA]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a0a ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a0a ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
IgE-mediated allergic response involves cross-linking of IgE bound on mast cells by specific surface epitopes of allergens. Structural studies on IgE epitopes of allergens are essential in understanding the characteristics of an allergen and for development of specific allergen immunotherapy. We have determined the structure of a group 13 dust mite allergen from Dermatophagoides farinae, Der f 13, using nuclear magnetic resonance. Sequence comparison of Der f 13 with homologous human fatty acid-binding proteins revealed unique surface charged residues on Der f 13 that may be involved in IgE binding and allergenicity. Site-directed mutagenesis and IgE binding assays have confirmed four surface charged residues on opposite sides of the protein that are involved in IgE binding. A triple mutant of Der f 13 (E41A_K63A_K91A) has been generated and found to have significantly reduced IgE binding and histamine release in skin prick tests on patients allergenic to group 13 dust mite allergens. The triple mutant is also able to induce PBMC proliferation in allergic patients with indices similar to those of wild-type Der f 13 and shift the secretion of cytokines from a Th2 to a Th1 pattern. Mouse IgG serum raised using the triple mutant is capable to block the binding of IgE from allergic patients to wild-type Der f 13, indicating potential for the triple mutant as a hypoallergen for specific immunotherapy. Findings in this study imply the importance of surface charged residues on IgE binding and allergenicity of an allergen, as was also demonstrated in other major allergens studied.
Nuclear magnetic resonance structure-based epitope mapping and modulation of dust mite group 13 allergen as a hypoallergen.,Chan SL, Ong ST, Ong SY, Chew FT, Mok YK J Immunol. 2006 Apr 15;176(8):4852-60. PMID:16585580<ref>PMID:16585580</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2a0a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: American house dust mite]]
[[Category: Dermatophagoides farinae]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chan, S L]]
[[Category: Chan SL]]
[[Category: Mok, Y K]]
[[Category: Mok YK]]
[[Category: Allergen]]
[[Category: Beta barrel]]
[[Category: Helix]]

Latest revision as of 06:37, 1 May 2024

Solution Structure of Der f 13, Group 13 Allergen from House Dust Mites

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