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<StructureSection load='2ckd' size='340' side='right'caption='[[2ckd]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='2ckd' size='340' side='right'caption='[[2ckd]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ckd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_leprae"_hansen_1880 "bacillus leprae" hansen 1880]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CKD FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ckd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CKD FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ckd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckd OCA], [https://pdbe.org/2ckd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ckd RCSB], [https://www.ebi.ac.uk/pdbsum/2ckd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckd ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ckd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ckd OCA], [https://pdbe.org/2ckd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ckd RCSB], [https://www.ebi.ac.uk/pdbsum/2ckd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ckd ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE]] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref>
[https://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ckd ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ckd ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacterium leprae protein ML2640c belongs to a large family of conserved hypothetical proteins predominantly found in mycobacteria, some of them predicted as putative S-adenosylmethionine (AdoMet)-dependent methyltransferases (MTase). As part of a Structural Genomics initiative on conserved hypothetical proteins in pathogenic mycobacteria, we have determined the structure of ML2640c in two distinct crystal forms. As expected, ML2640c has a typical MTase core domain and binds the methyl donor substrate AdoMet in a manner consistent with other known members of this structural family. The putative acceptor substrate-binding site of ML2640c is a large internal cavity, mostly lined by aromatic and aliphatic side-chain residues, suggesting that a lipid-like molecule might be targeted for catalysis. A flap segment (residues 222-256), which isolates the binding site from the bulk solvent and is highly mobile in the crystal structures, could serve as a gateway to allow substrate entry and product release. The multiple sequence alignment of ML2640c-like proteins revealed that the central alpha/beta core and the AdoMet-binding site are very well conserved within the family. However, the amino acid positions defining the binding site for the acceptor substrate display a higher variability, suggestive of distinct acceptor substrate specificities. The ML2640c crystal structures offer the first structural glimpses at this important family of mycobacterial proteins and lend strong support to their functional assignment as AdoMet-dependent methyltransferases.
The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria.,Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248<ref>PMID:17660248</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ckd" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus leprae hansen 1880]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Alzari, P M]]
[[Category: Mycobacterium leprae]]
[[Category: Buschiazzo, A]]
[[Category: Alzari PM]]
[[Category: Grana, M]]
[[Category: Buschiazzo A]]
[[Category: Haouz, A]]
[[Category: Grana M]]
[[Category: Wehenkel, A]]
[[Category: Haouz A]]
[[Category: Transferase]]
[[Category: Wehenkel A]]

Latest revision as of 06:39, 1 May 2024

Crystal structure of ML2640 from Mycobacterium leprae

2ckd, resolution 2.80Å

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