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==Solution NMR Structure of the replication Factor A Related Protein from Methanobacterium thermoautotrophicum. Northeast Structural Genomics Target TR91A.==
==Solution NMR Structure of the replication Factor A Related Protein from Methanobacterium thermoautotrophicum. Northeast Structural Genomics Target TR91A.==
<StructureSection load='2k50' size='340' side='right'caption='[[2k50]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2k50' size='340' side='right'caption='[[2k50]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2k50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metth Metth]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K50 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2k50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K50 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MTH1385, MTH_1385 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 METTH])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k50 OCA], [https://pdbe.org/2k50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k50 RCSB], [https://www.ebi.ac.uk/pdbsum/2k50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k50 ProSAT], [https://www.topsan.org/Proteins/NESGC/2k50 TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k50 OCA], [https://pdbe.org/2k50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k50 RCSB], [https://www.ebi.ac.uk/pdbsum/2k50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k50 ProSAT], [https://www.topsan.org/Proteins/NESGC/2k50 TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RPA_METTH RPA_METTH]] Inhibits DNA polymerase activity of PolB, which can be overcome by RFC and PNCA. Stimulates 3'-to 5'-exonuclease activity of PolB at 30 degrees Celsius, but has no effect at 50 or 70 degrees Celsius. Bind ssDNA and replication forks; replication forks structures bind both Hel308 and this protein. Has no effect on helicase activity of Hel308; may help target the helicase to DNA substrates that require DNA re-modeling.<ref>PMID:10497247</ref> <ref>PMID:21195035</ref>
[https://www.uniprot.org/uniprot/RPA_METTH RPA_METTH] Inhibits DNA polymerase activity of PolB, which can be overcome by RFC and PNCA. Stimulates 3'-to 5'-exonuclease activity of PolB at 30 degrees Celsius, but has no effect at 50 or 70 degrees Celsius. Bind ssDNA and replication forks; replication forks structures bind both Hel308 and this protein. Has no effect on helicase activity of Hel308; may help target the helicase to DNA substrates that require DNA re-modeling.<ref>PMID:10497247</ref> <ref>PMID:21195035</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Metth]]
[[Category: Methanothermobacter thermautotrophicus str. Delta H]]
[[Category: Acton, T B]]
[[Category: Acton TB]]
[[Category: Ciccosanti, C]]
[[Category: Ciccosanti C]]
[[Category: Everett, J K]]
[[Category: Everett JK]]
[[Category: Foote, E L]]
[[Category: Foote EL]]
[[Category: Jiang, M]]
[[Category: Jiang M]]
[[Category: Maglaqui, M]]
[[Category: Maglaqui M]]
[[Category: Montelione, G T]]
[[Category: Montelione GT]]
[[Category: Structural genomic]]
[[Category: Nair R]]
[[Category: Nair, R]]
[[Category: Rossi P]]
[[Category: Rossi, P]]
[[Category: Rost B]]
[[Category: Rost, B]]
[[Category: Swapna G]]
[[Category: Swapna, G]]
[[Category: Xiao R]]
[[Category: Xiao, R]]
[[Category: Nesg]]
[[Category: PSI, Protein structure initiative]]
[[Category: Uncharacterized protein]]
[[Category: Unknown function]]

Latest revision as of 06:44, 1 May 2024

Solution NMR Structure of the replication Factor A Related Protein from Methanobacterium thermoautotrophicum. Northeast Structural Genomics Target TR91A.

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