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==Refined solution structure of Methanosarcina thermophila protein MC1==
==Refined solution structure of Methanosarcina thermophila protein MC1==
<StructureSection load='2khl' size='340' side='right'caption='[[2khl]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
<StructureSection load='2khl' size='340' side='right'caption='[[2khl]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2khl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dsm_1825 Dsm 1825]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KHL FirstGlance]. <br>
<table><tr><td colspan='2'>[[2khl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KHL FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t23|1t23]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2khl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khl OCA], [https://pdbe.org/2khl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2khl RCSB], [https://www.ebi.ac.uk/pdbsum/2khl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2khl ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2khl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2khl OCA], [https://pdbe.org/2khl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2khl RCSB], [https://www.ebi.ac.uk/pdbsum/2khl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2khl ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/HMC1_METTE HMC1_METTE]] Protects DNA against thermal denaturation and modulates transcription.  
[https://www.uniprot.org/uniprot/HMC1_METTE HMC1_METTE] Protects DNA against thermal denaturation and modulates transcription.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2khl ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2khl ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 3D structure of methanogen chromosomal protein 1 (MC1), determined with heteronuclear NMR methods, agrees with its function in terms of the shape and nature of the binding surface, whereas the 3D structure determined with homonuclear NMR does not. The structure features five loops, which show a large distribution in the ensemble of 3D structures. Evidence for the fact that this distribution signifies internal mobility on the nanosecond time scale was provided by using (15)N-relaxation and molecular dynamics simulations. Structural variations of the arm (11 residues) induced large shape anisotropy variations on the nanosecond time scale that ruled out the use of the model-free formalism to analyze the relaxation data. The backbone dynamics analysis of MC1 was achieved by comparison with 20 ns molecular dynamics trajectories. Two beta-bulges showed that hydrogen bond formation correlated with varphi and psi dihedral angle transitions. These jumps were observed on the nanosecond time scale, in agreement with a large decrease in (15)N-NOE for Gly17 and Ile89. One water molecule bridging NH(Glu87) and CO(Val57) through hydrogen bonding contributed to these dynamics. Nanosecond slow motions observed in loops LP3 (35-42) and LP5 (67-77) reflected the lack of stable hydrogen bonds, whereas the other loops, LP1 (10-14), LP2 (22-24), and LP4 (50-53), were stabilized by several hydrogen bonds. Dynamics are often directly related to function. Our data strongly suggest that residues belonging to the flexible regions of MC1 could be involved in the interaction with DNA.
Refined solution structure and backbone dynamics of the archaeal MC1 protein.,Paquet F, Loth K, Meudal H, Culard F, Genest D, Lancelot G FEBS J. 2010 Dec;277(24):5133-45. doi: 10.1111/j.1742-4658.2010.07927.x., Epub 2010 Nov 16. PMID:21078128<ref>PMID:21078128</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2khl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dsm 1825]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Culard, F]]
[[Category: Methanosarcina thermophila]]
[[Category: Lancelot, G]]
[[Category: Culard F]]
[[Category: Meudal, H]]
[[Category: Lancelot G]]
[[Category: Paquet, F]]
[[Category: Meudal H]]
[[Category: Alpha and beta]]
[[Category: Paquet F]]
[[Category: Dna binding protein]]
[[Category: Dna-binding]]
[[Category: Dna-binding protein]]
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