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==NMR structure of the sea anemone actinoporin Sticholysin==
==NMR structure of the sea anemone actinoporin Sticholysin==
<StructureSection load='2ks4' size='340' side='right'caption='[[2ks4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2ks4' size='340' side='right'caption='[[2ks4]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stihl Stihl]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ks3|2ks3]], [[1kd6|1kd6]], [[1iaz|1iaz]], [[1gwy|1gwy]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 STIHL])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [https://pdbe.org/2ks4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [https://pdbe.org/2ks4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/ACTP1_STOHE ACTP1_STOHE]] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects (By similarity).  
[https://www.uniprot.org/uniprot/ACTP1_STIHL ACTP1_STIHL] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and cytolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects.<ref>PMID:10978735</ref> <ref>PMID:11478962</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ks4 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ks4 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sticholysin I is an actinoporin, a pore forming toxin, of 176 aminoacids produced by the sea anemone Stichodactyla heliantus. Isotopically labelled (13)C/(15)N recombinant protein was produced in E. coli. Here we report the complete NMR (15)N, (13)C and (1)H chemical shifts assignments of Stn I at pH 4.0 and 25 degrees C (BMRB No. 15927).
1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I.,Castrillo I, Alegre-Cebollada J, del Pozo AM, Gavilanes JG, Santoro J, Bruix M Biomol NMR Assign. 2009 Jun;3(1):5-7. Epub 2008 Nov 6. PMID:19636934<ref>PMID:19636934</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2ks4" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Stihl]]
[[Category: Stichodactyla helianthus]]
[[Category: Bruix, M]]
[[Category: Bruix M]]
[[Category: Castrillo, I]]
[[Category: Castrillo I]]
[[Category: Santoro, J]]
[[Category: Santoro J]]
[[Category: Cytolysis]]
[[Category: Hemolysis]]
[[Category: Ion transport]]
[[Category: Membrane]]
[[Category: Nematocyst]]
[[Category: Porin]]
[[Category: Protein]]
[[Category: Secreted]]
[[Category: Toxin]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Transport protein]]
Retrieved from "https://proteopedia.org/2ks4"