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==NMR solution structure of a soluble PrgI mutant from Salmonella Typhimurium==
==NMR solution structure of a soluble PrgI mutant from Salmonella Typhimurium==
<StructureSection load='2kv7' size='340' side='right'caption='[[2kv7]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
<StructureSection load='2kv7' size='340' side='right'caption='[[2kv7]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2kv7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salts Salts]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KV7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2kv7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._SL1344 Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KV7 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgI, STM2873 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216597 SALTS])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv7 OCA], [https://pdbe.org/2kv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kv7 RCSB], [https://www.ebi.ac.uk/pdbsum/2kv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kv7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv7 OCA], [https://pdbe.org/2kv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kv7 RCSB], [https://www.ebi.ac.uk/pdbsum/2kv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kv7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY]] Required for invasion of epithelial cells.  
[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY] Required for invasion of epithelial cells.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kv7 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kv7 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pathogenic Gram-negative bacteria use a type three secretion system (TTSS) to deliver virulence factors into host cells. Although the order in which proteins incorporate into the growing TTSS is well described, the underlying assembly mechanisms are still unclear. Here we show that the TTSS needle protomer refolds spontaneously to extend the needle from the distal end. We developed a functional mutant of the needle protomer from Shigella flexneri and Salmonella typhimurium to study its assembly in vitro. We show that the protomer partially refolds from alpha-helix into beta-strand conformation to form the TTSS needle. Reconstitution experiments show that needle growth does not require ATP. Thus, like the structurally related flagellar systems, the needle elongates by subunit polymerization at the distal end but requires protomer refolding. Our studies provide a starting point to understand the molecular assembly mechanisms and the structure of the TTSS at atomic level.
Protein refolding is required for assembly of the type three secretion needle.,Poyraz O, Schmidt H, Seidel K, Delissen F, Ader C, Tenenboim H, Goosmann C, Laube B, Thunemann AF, Zychlinsky A, Baldus M, Lange A, Griesinger C, Kolbe M Nat Struct Mol Biol. 2010 Jul;17(7):788-92. Epub 2010 Jun 13. PMID:20543831<ref>PMID:20543831</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kv7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Salts]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344]]
[[Category: Ader, C]]
[[Category: Ader C]]
[[Category: Baldus, M]]
[[Category: Baldus M]]
[[Category: Delissen, F]]
[[Category: Delissen F]]
[[Category: Goosmann, C]]
[[Category: Goosmann C]]
[[Category: Griesinger, C]]
[[Category: Griesinger C]]
[[Category: Kolbe, M]]
[[Category: Kolbe M]]
[[Category: Lange, A]]
[[Category: Lange A]]
[[Category: Laube, B]]
[[Category: Laube B]]
[[Category: Poyraz, O]]
[[Category: Poyraz O]]
[[Category: Schmidt, H]]
[[Category: Schmidt H]]
[[Category: Seidel, K]]
[[Category: Seidel K]]
[[Category: Tenenboim, H]]
[[Category: Tenenboim H]]
[[Category: Thuenemann, A F]]
[[Category: Thuenemann AF]]
[[Category: Zychlinski, A]]
[[Category: Zychlinski A]]
[[Category: Bacterial pathogenesis]]
[[Category: Needle protomer]]
[[Category: Prgi]]
[[Category: Protein transport]]
[[Category: Transport]]
[[Category: Type three secretion needle]]
[[Category: Virulence]]
Retrieved from "https://proteopedia.org/2kv7"