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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n4l OCA], [https://pdbe.org/2n4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n4l RCSB], [https://www.ebi.ac.uk/pdbsum/2n4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n4l ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n4l OCA], [https://pdbe.org/2n4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n4l RCSB], [https://www.ebi.ac.uk/pdbsum/2n4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n4l ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Splicing patterns in human immunodeficiency virus type 1 (HIV-1) are maintained through cis regulatory elements that recruit antagonistic host RNA-binding proteins. The activity of the 3' acceptor site A7 is tightly regulated through a complex network of an intronic splicing silencer (ISS), a bipartite exonic splicing silencer (ESS3a/b), and an exonic splicing enhancer (ESE3). Because HIV-1 splicing depends on protein-RNA interactions, it is important to know the tertiary structures surrounding the splice sites. Herein, we present the NMR solution structure of the phylogenetically conserved ISS stem loop. ISS adopts a stable structure consisting of conserved UG wobble pairs, a folded 2X2 (GU/UA) internal loop, a UU bulge, and a flexible AGUGA apical loop. Calorimetric and biochemical titrations indicate that the UP1 domain of heterogeneous nuclear ribonucleoprotein A1 binds the ISS apical loop site-specifically and with nanomolar affinity. Collectively, this work provides additional insights into how HIV-1 uses a conserved RNA structure to commandeer a host RNA-binding protein.
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| Solution Structure of the HIV-1 Intron Splicing Silencer and Its Interactions with the UP1 Domain of Heterogeneous Nuclear Ribonucleoprotein (hnRNP) A1.,Jain N, Morgan CE, Rife BD, Salemi M, Tolbert BS J Biol Chem. 2016 Jan 29;291(5):2331-44. doi: 10.1074/jbc.M115.674564. Epub 2015 , Nov 24. PMID:26607354<ref>PMID:26607354</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2n4l" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
