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==NMR Solution Structure of Mesoderm Development (MESD) - open conformation==
==NMR Solution Structure of Mesoderm Development (MESD) - open conformation==
<StructureSection load='2rqm' size='340' side='right'caption='[[2rqm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2rqm' size='340' side='right'caption='[[2rqm]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2rqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQM FirstGlance]. <br>
<table><tr><td colspan='2'>[[2rqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQM FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rqk|2rqk]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mesdc2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqm OCA], [https://pdbe.org/2rqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqm RCSB], [https://www.ebi.ac.uk/pdbsum/2rqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqm ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqm OCA], [https://pdbe.org/2rqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqm RCSB], [https://www.ebi.ac.uk/pdbsum/2rqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqm ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/MESD_MOUSE MESD_MOUSE]] Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.<ref>PMID:12581525</ref> <ref>PMID:21397183</ref>
[https://www.uniprot.org/uniprot/MESD_MOUSE MESD_MOUSE] Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction.<ref>PMID:12581525</ref> <ref>PMID:21397183</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rqm ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rqm ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mesoderm development (MESD) is a 224 amino acid mouse protein that acts as a molecular chaperone for the low-density lipoprotein receptor (LDLR) family. Here, we provide evidence that the region 45-184 of MESD is essential and sufficient for this function and suggest a model for its mode of action. NMR studies reveal a beta-alpha-beta-beta-alpha-beta core domain with an alpha-helical N-terminal extension that interacts with the beta sheet in a dynamic manner. As a result, the structural ensemble contains open (active) and closed (inactive) forms, allowing for regulation of chaperone activity through substrate binding. The mutant W61R, which is lethal in Drosophila, adopts only the open state. The receptor motif recognized by MESD was identified by in vitro-binding studies. Furthermore, in vivo functional evidence for the relevance of the identified contact sites in MESD is provided.
The Structure of MESD45-184 Brings Light into the Mechanism of LDLR Family Folding.,Kohler C, Lighthouse JK, Werther T, Andersen OM, Diehl A, Schmieder P, Du J, Holdener BC, Oschkinat H Structure. 2011 Mar 9;19(3):337-48. PMID:21397185<ref>PMID:21397185</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2rqm" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lk3 transgenic mice]]
[[Category: Mus musculus]]
[[Category: Andersen, O M]]
[[Category: Andersen OM]]
[[Category: Diehl, A]]
[[Category: Diehl A]]
[[Category: Holdener, B C]]
[[Category: Holdener BC]]
[[Category: Koehler, C]]
[[Category: Koehler C]]
[[Category: Lighthouse, J K]]
[[Category: Lighthouse JK]]
[[Category: Oschkinat, H]]
[[Category: Oschkinat H]]
[[Category: Schmieder, P]]
[[Category: Schmieder P]]
[[Category: Werther, T]]
[[Category: Werther T]]
[[Category: Chaperone]]

Latest revision as of 07:05, 1 May 2024

NMR Solution Structure of Mesoderm Development (MESD) - open conformation

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