2v8s: Difference between revisions

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<StructureSection load='2v8s' size='340' side='right'caption='[[2v8s]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
<StructureSection load='2v8s' size='340' side='right'caption='[[2v8s]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2v8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V8S FirstGlance]. <br>
<table><tr><td colspan='2'>[[2v8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V8S FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1xgw|1xgw]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8s OCA], [https://pdbe.org/2v8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v8s RCSB], [https://www.ebi.ac.uk/pdbsum/2v8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v8s ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8s OCA], [https://pdbe.org/2v8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v8s RCSB], [https://www.ebi.ac.uk/pdbsum/2v8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v8s ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/EPN4_HUMAN EPN4_HUMAN]] Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.<ref>PMID:12429846</ref> <ref>PMID:12538641</ref> [[https://www.uniprot.org/uniprot/VTI1B_HUMAN VTI1B_HUMAN]] V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.
[https://www.uniprot.org/uniprot/EPN4_HUMAN EPN4_HUMAN] Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.<ref>PMID:12429846</ref> <ref>PMID:12538641</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v8s ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v8s ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Soluble NSF attachment protein receptors (SNAREs) are type II transmembrane proteins that have critical roles in providing the specificity and energy for transport-vesicle fusion and must therefore be correctly partitioned between vesicle and organelle membranes. Like all other cargo, SNAREs need to be sorted into the forming vesicles by direct interaction with components of the vesicles' coats. Here we characterize the molecular details governing the sorting of a SNARE into clathrin-coated vesicles, namely the direct recognition of the three-helical bundle H(abc) domain of the mouse SNARE Vti1b by the human clathrin adaptor epsinR (EPNR, also known as CLINT1). Structures of each domain and of their complex show that this interaction (dissociation constant 22 muM) is mediated by surface patches composed of approximately 15 residues each, the topographies of which are dependent on each domain's overall fold. Disruption of the interface with point mutations abolishes the interaction in vitro and causes Vti1b to become relocalized to late endosomes and lysosomes. This new class of highly specific, surface-surface interaction between the clathrin coat component and the cargo is distinct from the widely observed binding of short, linear cargo motifs by the assembly polypeptide (AP) complex and GGA adaptors and is therefore not vulnerable to competition from standard motif-containing cargoes for incorporation into clathrin-coated vesicles. We propose that conceptually similar but mechanistically different interactions will direct the post-Golgi trafficking of many SNAREs.
A SNARE-adaptor interaction is a new mode of cargo recognition in clathrin-coated vesicles.,Miller SE, Collins BM, McCoy AJ, Robinson MS, Owen DJ Nature. 2007 Nov 22;450(7169):570-4. PMID:18033301<ref>PMID:18033301</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2v8s" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Collins, B M]]
[[Category: Collins BM]]
[[Category: McCoy, A J]]
[[Category: McCoy AJ]]
[[Category: Miller, S E]]
[[Category: Miller SE]]
[[Category: Owen, D J]]
[[Category: Owen DJ]]
[[Category: Adaptor]]
[[Category: Alternative splicing]]
[[Category: Cargo]]
[[Category: Clathrin]]
[[Category: Coiled coil]]
[[Category: Cytoplasm]]
[[Category: Cytoplasmic vesicle]]
[[Category: Endocytosis]]
[[Category: Lipid-binding]]
[[Category: Membrane]]
[[Category: Phosphorylation]]
[[Category: Protein transport]]
[[Category: Snare]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Vesicle]]
[[Category: Vti1b-epsinr complex]]

Latest revision as of 07:07, 1 May 2024

VTI1B HABC DOMAIN - EPSINR ENTH DOMAIN COMPLEX

2v8s, resolution 2.22Å

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