2vuv: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2vuv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Codakia_orbicularis Codakia orbicularis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUV FirstGlance]. <br>
<table><tr><td colspan='2'>[[2vuv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Codakia_orbicularis Codakia orbicularis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vuz|2vuz]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vuv OCA], [https://pdbe.org/2vuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vuv RCSB], [https://www.ebi.ac.uk/pdbsum/2vuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vuv ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vuv OCA], [https://pdbe.org/2vuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vuv RCSB], [https://www.ebi.ac.uk/pdbsum/2vuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vuv ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q3KVL7_9BIVA Q3KVL7_9BIVA]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vuv ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vuv ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Codakine is an abundant 14-kDa mannose-binding C-type lectin isolated from the gills of the sea bivalve Codakia orbicularis. Binding studies using inhibition of hemagglutination indicated specificity for mannose and fucose monosaccharides. Further experiments using a glycan array demonstrated, however, a very fine specificity for N-linked biantennary complex-type glycans. An unusually high affinity was measured by titration microcalorimetry performed with a biantennary Asn-linked nonasaccharide. The crystal structure of the native lectin at 1.3A resolution revealed a new type of disulfide-bridged homodimer. Each monomer displays three intramolecular disulfide bridges and contains only one calcium ion located in the canonical binding site that is occupied by a glycerol molecule. The structure of the complex between Asn-linked nonasaccharide and codakine has been solved at 1.7A resolution. All residues could be located in the electron density map, except for the capping beta1-4-linked galactosides. The alpha1-6-linked mannose binds to calcium by coordinating the O3 and O4 hydroxyl groups. The GlcNAc moiety of the alpha1,6 arm engages in several hydrogen bonds with the protein, whereas the GlcNAc on the other antenna is stacked against Trp(108), forming an extended binding site. This is the first structural report for a bivalve lectin.
High affinity interaction between a bivalve C-type lectin and a biantennary complex-type N-glycan revealed by crystallography and microcalorimetry.,Gourdine JP, Cioci G, Miguet L, Unverzagt C, Silva DV, Varrot A, Gautier C, Smith-Ravin EJ, Imberty A J Biol Chem. 2008 Oct 31;283(44):30112-20. Epub 2008 Aug 7. PMID:18687680<ref>PMID:18687680</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2vuv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Codakia orbicularis]]
[[Category: Codakia orbicularis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cioci, G C]]
[[Category: Cioci GC]]
[[Category: Gauthier, C]]
[[Category: Gauthier C]]
[[Category: Gourdine, J P]]
[[Category: Gourdine JP]]
[[Category: Imberty, A]]
[[Category: Imberty A]]
[[Category: Miguet, L]]
[[Category: Miguet L]]
[[Category: Smith-Ravin, E J]]
[[Category: Smith-Ravin EJ]]
[[Category: Unverzagt, C]]
[[Category: Unverzagt C]]
[[Category: Varrot, A]]
[[Category: Varrot A]]
[[Category: C-type]]
[[Category: Codakine]]
[[Category: Invertebrate]]
[[Category: Lectin]]
[[Category: Mannose]]
[[Category: Sugar binding protein]]
[[Category: Sugar-binding protein]]
Retrieved from "https://proteopedia.org/2vuv"