2w4s: Difference between revisions

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<StructureSection load='2w4s' size='340' side='right'caption='[[2w4s]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
<StructureSection load='2w4s' size='340' side='right'caption='[[2w4s]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2w4s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum_iowa Cryptosporidium parvum iowa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W4S FirstGlance]. <br>
<table><tr><td colspan='2'>[[2w4s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum_Iowa Cryptosporidium parvum Iowa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W4S FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w4s OCA], [https://pdbe.org/2w4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w4s RCSB], [https://www.ebi.ac.uk/pdbsum/2w4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w4s ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w4s OCA], [https://pdbe.org/2w4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w4s RCSB], [https://www.ebi.ac.uk/pdbsum/2w4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w4s ProSAT]</span></td></tr>
</table>
</table>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w4s ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w4s ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Small nuclear and small nucleolar RNAs (snRNAs and snoRNAs) are critical components of snRNPs and snoRNPs and play an essential role in the maturation of, respectively, mRNAs and rRNAs within the nucleus of eukaryotic cells. Complex and specific pathways exist for the assembly of snRNPs and snoRNPs, involving, for instance, nucleocytoplasmic transport of snRNAs and intranuclear transport between compartments of snoRNAs. The phosphorylated adaptor for nuclear export (PHAX) is required for nuclear export of snRNAs in metazoans and also involved in the intranuclear transport of snoRNAs to Cajal bodies. PHAX contains a conserved single-stranded nucleic acid binding domain (RNA_GG_bind domain) with no sequence homology with any other known RNA-binding module. Here, we report NMR and X-ray crystallography studies that elucidate the structural basis for RNA recognition by the PHAX RNA-binding domain (PHAX-RBD). The crystal structure of the RNA_GG_bind domain from the parasite Cryptosporidium parvum (Cp RBD) forms well-folded dimers in solution in the absence of any ligand. The human PHAX-RBD is monomeric and only adopts a tertiary fold upon RNA binding. The PHAX-RBD represents a novel helical fold and binds single-stranded RNA with micromolar affinity without sequence specificity. RNA recognition by human PHAX-RBD is consistent with mutational analysis that affects RNA binding and PHAX-mediated nuclear export. Our data suggest that the PHAX-RBD mediates auxiliary RNA contacts with the snRNA and snoRNA substrates that are required for transport and/or substrate release.
Structure and RNA recognition by the snRNA and snoRNA transport factor PHAX.,Mourao A, Varrot A, Mackereth CD, Cusack S, Sattler M RNA. 2010 Jun;16(6):1205-16. Epub 2010 Apr 29. PMID:20430857<ref>PMID:20430857</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2w4s" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cryptosporidium parvum iowa]]
[[Category: Cryptosporidium parvum Iowa]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cusack, S]]
[[Category: Cusack S]]
[[Category: Mackereth, C]]
[[Category: Mackereth C]]
[[Category: Mourao, A]]
[[Category: Mourao A]]
[[Category: Sattler, M]]
[[Category: Sattler M]]
[[Category: Varrot, A]]
[[Category: Varrot A]]
[[Category: Rna transport]]
[[Category: Structural protein]]

Latest revision as of 07:09, 1 May 2024

novel RNA-binding domain in Cryptosporidium parvum at 2.5 angstrom resolution

2w4s, resolution 2.45Å

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