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| == Function == | | == Function == |
| [https://www.uniprot.org/uniprot/MB211_HUMAN MB211_HUMAN] Required for several aspects of embryonic development including normal development of the eye. | | [https://www.uniprot.org/uniprot/MB211_HUMAN MB211_HUMAN] Required for several aspects of embryonic development including normal development of the eye. |
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| == Publication Abstract from PubMed ==
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| The exceptionally conserved metazoan MAB21 proteins are implicated in cell fate decisions and share considerable sequence homology with the cyclic GMP-AMP synthase. cGAS is the major innate immune sensor for cytosolic DNA and produces the second messenger 2'-5', 3'-5' cyclic GMP-AMP. Little is known about the structure and biochemical function of other proteins of the cGAS-MAB21 subfamily, such as MAB21L1, MAB21L2 and MAB21L3. We have determined the crystal structure of human full-length MAB21L1. Our analysis reveals high structural conservation between MAB21L1 and cGAS but also uncovers important differences. Although monomeric in solution, MAB21L1 forms a highly symmetric double-pentameric oligomer in the crystal, raising the possibility that oligomerization could be a feature of MAB21L1. In the crystal, MAB21L1 is in an inactive conformation requiring a conformational change - similar to cGAS - to develop any nucleotidyltransferase activity. Co-crystallization with NTP identified a putative ligand binding site of MAB21 proteins that corresponds to the DNA binding site of cGAS. Finally, we offer a structure-based explanation for the effects of MAB21L2 mutations in patients with eye malformations. The underlying residues participate in fold-stabilizing interaction networks and mutations destabilize the protein. In summary, we provide a first structural framework for MAB21 proteins.
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| Structural and biochemical characterization of the cell fate determining nucleotidyltransferase fold protein MAB21L1.,de Oliveira Mann CC, Kiefersauer R, Witte G, Hopfner KP Sci Rep. 2016 Jun 8;6:27498. doi: 10.1038/srep27498. PMID:27271801<ref>PMID:27271801</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5eom" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
