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==Pholiota squarrosa lectin trimer==
==Pholiota squarrosa lectin trimer==
<StructureSection load='5xzk' size='340' side='right'caption='[[5xzk]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='5xzk' size='340' side='right'caption='[[5xzk]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5xzk]] is a 3 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XZK FirstGlance]. <br>
<table><tr><td colspan='2'>[[5xzk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pholiota_squarrosa Pholiota squarrosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XZK FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xzk OCA], [http://pdbe.org/5xzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xzk RCSB], [http://www.ebi.ac.uk/pdbsum/5xzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xzk ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xzk OCA], [https://pdbe.org/5xzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xzk RCSB], [https://www.ebi.ac.uk/pdbsum/5xzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xzk ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A384E107_9AGAR A0A384E107_9AGAR]
The core alpha1-6 fucosylation-specific lectin from a mushroom Pholiota squarrosa (PhoSL) is a potential tool for precise diagnosis of cancers. This lectin consists of only 40 amino acids and can be chemically synthesized. We showed here that a synthesized PhoSL peptide formed a trimer by gel filtration and chemical cross-linking assays, and determined a structure of the PhoSL trimer by NMR. The structure possesses a beta-prism motif with a three-fold rotational symmetry, where three antiparallel beta-sheets are tightly connected by swapping of beta-strands. A triad of Trp residues comprises the structural core, forming NH-pi electrostatic interactions among the indole rings. NMR analysis with an excess amount of fucose revealed the structural basis for the molecular recognition. Namely, fucose deeply enters a pocket formed at a junction of beta-sheet edges, with the methyl group placed at the bottom. It forms a number of hydrophobic and hydrogen-bonding interactions with PhoSL residues. In spite of partial similarities to the structures of other functionally related lectins, the arrangement of the antiparallel beta-sheets in the PhoSL trimer is novel as a structural scaffold, and thus defines a novel type of lectin structure.
 
The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL.,Yamasaki K, Yamasaki T, Tateno H Sci Rep. 2018 May 17;8(1):7740. doi: 10.1038/s41598-018-25630-2. PMID:29773815<ref>PMID:29773815</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xzk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Yamasaki, K]]
[[Category: Pholiota squarrosa]]
[[Category: Fucose]]
[[Category: Yamasaki K]]
[[Category: Lectin]]
[[Category: Sugar binding protein]]
[[Category: Trimer]]

Latest revision as of 07:28, 1 May 2024

Pholiota squarrosa lectin trimer

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