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==HYBRID NMR/CRYO-EM STRUCTURE OF THE HIV-1 RNA DIMERIZATION SIGNAL==
==HYBRID NMR/CRYO-EM STRUCTURE OF THE HIV-1 RNA DIMERIZATION SIGNAL==
<SX load='6bg9' size='340' side='right' viewer='molstar' caption='[[6bg9]], [[Resolution|resolution]] 9.00&Aring;, [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<SX load='6bg9' size='340' side='right' viewer='molstar' caption='[[6bg9]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6bg9]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BG9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BG9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6bg9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BG9 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bg9 OCA], [http://pdbe.org/6bg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6bg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bg9 ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , Solution NMR, [[Resolution|Resolution]] 9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bg9 OCA], [https://pdbe.org/6bg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bg9 RCSB], [https://www.ebi.ac.uk/pdbsum/6bg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bg9 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cryoelectron microscopy (cryo-EM) and nuclear magnetic resonance (NMR) spectroscopy are routinely used to determine structures of macromolecules with molecular weights over 65 and under 25 kDa, respectively. We combined these techniques to study a 30 kDa HIV-1 dimer initiation site RNA ([DIS]2; 47 nt/strand). A 9 A cryo-EM map clearly shows major groove features of the double helix and a right-handed superhelical twist. Simulated cryo-EM maps generated from time-averaged molecular dynamics trajectories (10 ns) exhibited levels of detail similar to those in the experimental maps, suggesting internal structural flexibility limits the cryo-EM resolution. Simultaneous inclusion of the cryo-EM map and (2)H-edited NMR-derived distance restraints during structure refinement generates a structure consistent with both datasets and supporting a flipped-out base within a conserved purine-rich bulge. Our findings demonstrate the power of combining global and local structural information from these techniques for structure determination of modest-sized RNAs.
Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR, and Molecular Dynamics Approach.,Zhang K, Keane SC, Su Z, Irobalieva RN, Chen M, Van V, Sciandra CA, Marchant J, Heng X, Schmid MF, Case DA, Ludtke SJ, Summers MF, Chiu W Structure. 2018 Feb 1. pii: S0969-2126(18)30001-7. doi:, 10.1016/j.str.2018.01.001. PMID:29398526<ref>PMID:29398526</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6bg9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</SX>
</SX>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Summers, M F]]
[[Category: Summers MF]]
[[Category: Gu wobble]]
[[Category: Rna]]
[[Category: Rna inernal loop]]
[[Category: S-turn thermodynamic]]
[[Category: Sheared ga pair]]

Latest revision as of 07:29, 1 May 2024

HYBRID NMR/CRYO-EM STRUCTURE OF THE HIV-1 RNA DIMERIZATION SIGNAL

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