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| ==Structure of an intra-locked G-quadruplex== | | ==Structure of an intra-locked G-quadruplex== |
| <StructureSection load='6kvb' size='340' side='right'caption='[[6kvb]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | | <StructureSection load='6kvb' size='340' side='right'caption='[[6kvb]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[6kvb]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KVB FirstGlance]. <br> | | <table><tr><td colspan='2'>[[6kvb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KVB FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvb OCA], [http://pdbe.org/6kvb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kvb RCSB], [http://www.ebi.ac.uk/pdbsum/6kvb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvb ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvb OCA], [https://pdbe.org/6kvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kvb RCSB], [https://www.ebi.ac.uk/pdbsum/6kvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvb ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| G-rich DNA sequences with tracts of three or more continuous guanines (G>/=3) are known to have high propensity to adopt stable G-quadruplex (G4) structures. Bioinformatic analyses suggest high prevalence of G-rich sequences with short G-tracts (G</=2) in the human genome. However, due to limited structural studies, the folding principles of such sequences remain largely unexplored and hence poorly understood. Here, we present the solution NMR structure of a sequence named AT26 consisting of irregularly spaced G2 tracts and two isolated single guanines. The structure is a four-layered G4 featuring two bi-layered blocks, locked between themselves in an unprecedented fashion making it a stable scaffold. In addition to edgewise and propeller-type loops, AT26 also harbors two V-shaped loops: a 2-nt V-shaped loop spanning two G-tetrad layers and a 0-nt V-shaped loop spanning three G-tetrad layers, which are named as VS- and VR-loop respectively, based on their distinct structural features. The intra-lock motif can be a basis for extending the G-tetrad core and a very stable intra-locked G4 can be formed by a sequence with G-tracts of various lengths including several G2 tracts. Findings from this study will aid in understanding the folding of G4 topologies from sequences containing irregularly spaced multiple short G-tracts.
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| Intra-locked G-quadruplex structures formed by irregular DNA G-rich motifs.,Maity A, Winnerdy FR, Chang WD, Chen G, Phan AT Nucleic Acids Res. 2020 Feb 26. pii: 5758051. doi: 10.1093/nar/gkaa008. PMID:32100003<ref>PMID:32100003</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 6kvb" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Chang, W D]] | | [[Category: Synthetic construct]] |
| [[Category: Chen, G]] | | [[Category: Chang WD]] |
| [[Category: Maity, A]] | | [[Category: Chen G]] |
| [[Category: Phan, A T]] | | [[Category: Maity A]] |
| [[Category: Winnerdy, F R]] | | [[Category: Phan AT]] |
| [[Category: Dna]] | | [[Category: Winnerdy FR]] |
| [[Category: Dna g-quadruplex]]
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| [[Category: Intra-locked]]
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| [[Category: Irregular g-rich motif]]
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| [[Category: Nmr spectroscopy]]
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