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<StructureSection load='6tjp' size='340' side='right'caption='[[6tjp]], [[Resolution|resolution]] 3.74&Aring;' scene=''>
<StructureSection load='6tjp' size='340' side='right'caption='[[6tjp]], [[Resolution|resolution]] 3.74&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6tjp]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TJP FirstGlance]. <br>
<table><tr><td colspan='2'>[[6tjp]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TJP FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6qwp|6qwp]], [[6qx5|6qx5]], [[6qxm|6qxm]], [[6r21|6r21]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.74&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tjp OCA], [https://pdbe.org/6tjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tjp RCSB], [https://www.ebi.ac.uk/pdbsum/6tjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tjp ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tjp OCA], [https://pdbe.org/6tjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tjp RCSB], [https://www.ebi.ac.uk/pdbsum/6tjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tjp ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PORTL_BPT7 PORTL_BPT7]] Forms the portal vertex of the capsid. This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel.[HAMAP-Rule:MF_04120]<ref>PMID:15784250</ref> <ref>PMID:23884409</ref>
[https://www.uniprot.org/uniprot/PORTL_BPT7 PORTL_BPT7] Forms the portal vertex of the capsid. This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel.[HAMAP-Rule:MF_04120]<ref>PMID:15784250</ref> <ref>PMID:23884409</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Medium-resolution cryo-electron microscopy maps, in particular when they include a significant number of alpha-helices, may allow the building of partial models that are useful for molecular-replacement searches in large crystallographic structures when the structures of homologs are not available and experimental phasing has failed. Here, as an example, the solution of the structure of a bacteriophage portal using a partial 30% model built into a 7.8 A resolution cryo-EM map is shown. Inspection of the self-rotation function allowed the correct oligomerization state to be determined, and density-modification procedures using rotation matrices and a mask based on the cryo-EM structure were critical for solving the structure. A workflow is described that may be applicable to similar cases and this strategy is compared with direct use of the cryo-EM map for molecular replacement.


Using a partial atomic model from medium-resolution cryo-EM to solve a large crystal structure.,Fabrega-Ferrer M, Cuervo A, Fernandez FJ, Machon C, Perez-Luque R, Pous J, Vega MC, Carrascosa JL, Coll M Acta Crystallogr D Struct Biol. 2021 Jan 1;77(Pt 1):11-18. doi:, 10.1107/S2059798320015156. Epub 2021 Jan 1. PMID:33404521<ref>PMID:33404521</ref>
==See Also==
 
*[[Portal protein 3D structures|Portal protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tjp" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bpt7]]
[[Category: Escherichia phage T7]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Carrascosa, J L]]
[[Category: Carrascosa JL]]
[[Category: Coll, M]]
[[Category: Coll M]]
[[Category: Cuervo, A]]
[[Category: Cuervo A]]
[[Category: Fabrega-Ferrer, M]]
[[Category: Fabrega-Ferrer M]]
[[Category: Fernandez, F J]]
[[Category: Fernandez FJ]]
[[Category: Machon, C]]
[[Category: Machon C]]
[[Category: Perez-Luque, R]]
[[Category: Perez-Luque R]]
[[Category: Pous, J]]
[[Category: Pous J]]
[[Category: Vega, M C]]
[[Category: Vega MC]]
[[Category: Dna packaging]]
[[Category: Viral protein]]

Latest revision as of 07:38, 1 May 2024

Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve - P212121

6tjp, resolution 3.74Å

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