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==IRES-targeting Small Molecule Inhibits Enterovirus 71 Replication via Allosteric Stabilization of a Ternary Complex==
==IRES-targeting Small Molecule Inhibits Enterovirus 71 Replication via Allosteric Stabilization of a Ternary Complex==
<StructureSection load='6xb7' size='340' side='right'caption='[[6xb7]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='6xb7' size='340' side='right'caption='[[6xb7]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6xb7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XB7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XB7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6xb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterovirus_A71 Enterovirus A71]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XB7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UYS:3-amino-N-(diaminomethylidene)-5-(dimethylamino)-6-(phenylethynyl)pyrazine-2-carboxamide'>UYS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xb7 OCA], [http://pdbe.org/6xb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xb7 RCSB], [http://www.ebi.ac.uk/pdbsum/6xb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xb7 ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UYS:3-amino-N-(diaminomethylidene)-5-(dimethylamino)-6-(phenylethynyl)pyrazine-2-carboxamide'>UYS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xb7 OCA], [https://pdbe.org/6xb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6xb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xb7 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enterovirus 71 (EV71) poses serious threats to human health, particularly in Southeast Asia, and no drugs or vaccines are available. Previous work identified the stem loop II structure of the EV71 internal ribosomal entry site as vital to viral translation and a potential target. After screening an RNA-biased library using a peptide-displacement assay, we identify DMA-135 as a dose-dependent inhibitor of viral translation and replication with no significant toxicity in cell-based studies. Structural, biophysical, and biochemical characterization support an allosteric mechanism in which DMA-135 induces a conformational change in the RNA structure that stabilizes a ternary complex with the AUF1 protein, thus repressing translation. This mechanism is supported by pull-down experiments in cell culture. These detailed studies establish enterovirus RNA structures as promising drug targets while revealing an approach and mechanism of action that should be broadly applicable to functional RNA targeting.
IRES-targeting small molecule inhibits enterovirus 71 replication via allosteric stabilization of a ternary complex.,Davila-Calderon J, Patwardhan NN, Chiu LY, Sugarman A, Cai Z, Penutmutchu SR, Li ML, Brewer G, Hargrove AE, Tolbert BS Nat Commun. 2020 Sep 22;11(1):4775. doi: 10.1038/s41467-020-18594-3. PMID:32963221<ref>PMID:32963221</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6xb7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Enterovirus A71]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chiu, L Y]]
[[Category: Chiu LY]]
[[Category: Davila, J]]
[[Category: Davila J]]
[[Category: Sugarman, A L]]
[[Category: Sugarman AL]]
[[Category: Tolbert, B S]]
[[Category: Tolbert BS]]
[[Category: Rna]]
[[Category: Rna binding protein]]
[[Category: Rna-small molecule complex]]

Latest revision as of 07:39, 1 May 2024

IRES-targeting Small Molecule Inhibits Enterovirus 71 Replication via Allosteric Stabilization of a Ternary Complex

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