6znh: Difference between revisions

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<StructureSection load='6znh' size='340' side='right'caption='[[6znh]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<StructureSection load='6znh' size='340' side='right'caption='[[6znh]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6znh]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZNH FirstGlance]. <br>
<table><tr><td colspan='2'>[[6znh]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZNH FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6znh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6znh OCA], [https://pdbe.org/6znh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6znh RCSB], [https://www.ebi.ac.uk/pdbsum/6znh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6znh ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6znh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6znh OCA], [https://pdbe.org/6znh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6znh RCSB], [https://www.ebi.ac.uk/pdbsum/6znh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6znh ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY]] Required for invasion of epithelial cells.  
[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY] Required for invasion of epithelial cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of Salmonella PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.
 
Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.,Kotov V, Lunelli M, Wald J, Kolbe M, Marlovits TC Biochem Biophys Rep. 2021 Jun 27;27:101039. doi: 10.1016/j.bbrep.2021.101039., eCollection 2021 Sep. PMID:34258394<ref>PMID:34258394</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6znh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Salmonella typhimurium]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Kolbe, M]]
[[Category: Kolbe M]]
[[Category: Kotov, V]]
[[Category: Kotov V]]
[[Category: Lunelli, M]]
[[Category: Lunelli M]]
[[Category: Marlovits, T C]]
[[Category: Marlovits TC]]
[[Category: Wald, J]]
[[Category: Wald J]]
[[Category: Helical reconstruction]]
[[Category: Needle filament]]
[[Category: Protein transport]]
[[Category: Salmonella]]
[[Category: Type 3 secretion system]]
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