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==Solution Structure of Human alpha-Parvalbumin (Minimized Average Structure)==
==Solution Structure of Human alpha-Parvalbumin (Minimized Average Structure)==
<StructureSection load='1rk9' size='340' side='right'caption='[[1rk9]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='1rk9' size='340' side='right'caption='[[1rk9]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RK9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RK9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rk9 OCA], [https://pdbe.org/1rk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rk9 RCSB], [https://www.ebi.ac.uk/pdbsum/1rk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rk9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rk9 OCA], [https://pdbe.org/1rk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rk9 RCSB], [https://www.ebi.ac.uk/pdbsum/1rk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rk9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/PRVA_HUMAN PRVA_HUMAN]] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.  
[https://www.uniprot.org/uniprot/PRVA_HUMAN PRVA_HUMAN] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rk9 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rk9 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin.,Baig I, Bertini I, Del Bianco C, Gupta YK, Lee YM, Luchinat C, Quattrone A Biochemistry. 2004 May 11;43(18):5562-73. PMID:15122922<ref>PMID:15122922</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1rk9" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Parvalbumin|Parvalbumin]]
*[[Parvalbumin|Parvalbumin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Baig, I]]
[[Category: Baig I]]
[[Category: Bertini, I]]
[[Category: Bertini I]]
[[Category: Bianco, C Del]]
[[Category: Del Bianco C]]
[[Category: Gupta, Y K]]
[[Category: Gupta YK]]
[[Category: Lee, Y M]]
[[Category: Lee Y-M]]
[[Category: Luchinat, C]]
[[Category: Luchinat C]]
[[Category: Quattrone, A]]
[[Category: Quattrone A]]
[[Category: SPINE, Structural Proteomics in Europe]]
[[Category: Calcium]]
[[Category: Ef-hand]]
[[Category: Lanthanide]]
[[Category: Metal binding protein]]
[[Category: Parvalbumin]]
[[Category: Spine]]
[[Category: Structural genomic]]
[[Category: Structural proteomics in europe]]
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