1rl1: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:


==Solution structure of human Sgt1 CS domain==
==Solution structure of human Sgt1 CS domain==
<StructureSection load='1rl1' size='340' side='right'caption='[[1rl1]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='1rl1' size='340' side='right'caption='[[1rl1]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1rl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RL1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1rl1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RL1 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SUGT1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rl1 OCA], [https://pdbe.org/1rl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rl1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rl1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rl1 OCA], [https://pdbe.org/1rl1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rl1 RCSB], [https://www.ebi.ac.uk/pdbsum/1rl1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rl1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/SUGT1_HUMAN SUGT1_HUMAN]] May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.  
[https://www.uniprot.org/uniprot/SGT1_HUMAN SGT1_HUMAN] May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Line 19: Line 19:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rl1 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rl1 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies.
Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain.,Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:14761955<ref>PMID:14761955</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1rl1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chazin, W J]]
[[Category: Chazin WJ]]
[[Category: Jacob, J]]
[[Category: Jacob J]]
[[Category: Kuznicki, J]]
[[Category: Kuznicki J]]
[[Category: Lee, Y T]]
[[Category: Lee Y-T]]
[[Category: Michowski, W]]
[[Category: Michowski W]]
[[Category: Nowotny, M]]
[[Category: Nowotny M]]
[[Category: Beta strand]]
[[Category: Beta sandwich]]
[[Category: Lacking last beta strand seen in p23]]
[[Category: Protein degradation]]
[[Category: Similar to p23]]

Revision as of 08:24, 1 May 2024

Solution structure of human Sgt1 CS domain

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1rl1&oldid=4139911"