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==Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)==
==Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)==
<StructureSection load='1s6i' size='340' side='right'caption='[[1s6i]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
<StructureSection load='1s6i' size='340' side='right'caption='[[1s6i]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1s6i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_hispida Glycine hispida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S6I FirstGlance]. <br>
<table><tr><td colspan='2'>[[1s6i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S6I FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1s6j|1s6j]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CDPK SK5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s6i OCA], [https://pdbe.org/1s6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s6i RCSB], [https://www.ebi.ac.uk/pdbsum/1s6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s6i ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s6i OCA], [https://pdbe.org/1s6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s6i RCSB], [https://www.ebi.ac.uk/pdbsum/1s6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s6i ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/CDPK_SOYBN CDPK_SOYBN]] May play a role in signal transduction pathways that involve calcium as a second messenger.  
[https://www.uniprot.org/uniprot/CDPK_SOYBN CDPK_SOYBN] May play a role in signal transduction pathways that involve calcium as a second messenger.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s6i ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s6i ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Calcium-dependent protein kinases (CDPKs) are an extensive class of multidomain Ca(2+)-regulated enzymes from plants and protozoa. In vivo the so-called calmodulin-like domain (CLD) of CDPK binds intramolecularly to the junction domain (JD), which exhibits both kinase-inhibitory and CLD binding properties. Here we report the high resolution solution structure of the calcium-regulatory region from soybean CDPK-alpha determined in the presence of a peptide encompassing the JD. The structure of both lobes of CLD resembles that of related helix-loop-helix Ca(2+)-binding proteins. NMR chemical shift mapping studies demonstrate that the JD induces significant structural changes in isolated Ca(2+)-CLD, particularly the C-terminal domain, although a stable complex is not formed. A CLD solution structure calculated on the basis of NMR data and long range fluorescence resonance energy transfer distances reveals an activated state with both lobes positioned side by side, similar to calcineurin B rather than calmodulin, highlighting the possible pitfall of assigning function purely from sequence information.
Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha.,Weljie AM, Vogel HJ J Biol Chem. 2004 Aug 20;279(34):35494-502. Epub 2004 May 20. PMID:15155727<ref>PMID:15155727</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1s6i" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Calcium-dependent protein kinase|Calcium-dependent protein kinase]]
*[[Calcium-dependent protein kinase|Calcium-dependent protein kinase]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Glycine hispida]]
[[Category: Glycine max]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Vogel, H J]]
[[Category: Vogel HJ]]
[[Category: Weljie, A M]]
[[Category: Weljie AM]]
[[Category: Calcium-binding]]
[[Category: Calmodulin superfamily]]
[[Category: Ef-hand]]
[[Category: Helix-loop-helix]]
[[Category: Plant protein]]
[[Category: Transferase]]

Revision as of 08:29, 1 May 2024

Ca2+-regulatory region (CLD) from soybean calcium-dependent protein kinase-alpha (CDPK) in the presence of Ca2+ and the junction domain (JD)

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