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==SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)==
==SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)==
<StructureSection load='1sxm' size='340' side='right'caption='[[1sxm]], [[NMR_Ensembles_of_Models | 39 NMR models]]' scene=''>
<StructureSection load='1sxm' size='340' side='right'caption='[[1sxm]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1sxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXM FirstGlance]. <br>
<table><tr><td colspan='2'>[[1sxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXM FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxm OCA], [https://pdbe.org/1sxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxm RCSB], [https://www.ebi.ac.uk/pdbsum/1sxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxm ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxm OCA], [https://pdbe.org/1sxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxm RCSB], [https://www.ebi.ac.uk/pdbsum/1sxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxm ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/KAX21_CENNO KAX21_CENNO]] Blocks voltage-gated non-inactivating potassium channels and unblocks inactivating potassium channels blocked by alpha-dendrotoxin in synaptosomes. Also displaces the alpha-dendrotoxin homolog dendrotoxin I from its receptor on brain synaptic membranes.  
[https://www.uniprot.org/uniprot/KAX21_CENNO KAX21_CENNO] Blocks voltage-gated non-inactivating potassium channels and unblocks inactivating potassium channels blocked by alpha-dendrotoxin in synaptosomes. Also displaces the alpha-dendrotoxin homolog dendrotoxin I from its receptor on brain synaptic membranes.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sxm ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sxm ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The 3D structure of noxiustoxin, the first identified scorpion toxin acting on K+ channels, has been elucidated by NMR and molecular modeling. Thirty-nine solution structures were calculated using 572 distance and 42 dihedral restraints. The average atomic rms deviation between the refined structures and the mean structure is 0.75 A for the backbone atoms. Noxiustoxin adopts a alpha/beta scaffold constituted of a three-stranded beta-sheet (residues 2-3, 25-30, 33-38) linked to a helix (residues 10-20) through two disulfide bridges. A comparison between the 3D structure of noxiustoxin and those of other structurally and functionally related scorpion toxins (charybdotoxin, PO5-NH2, kaliotoxin) revealed a bending capacity of the helix and a variability in the relative orientations between the helix and the beta-sheet. These two features highlight the plasticity of the alpha/beta scaffold and offer a structural explanation for the capacity of the fold to accommodate an additional alanine residue in the Gly-x-Cys pattern of a previously proposed consensus sequence [Bontems et al. (1991) Science 254, 1521-1523]. Our structural data also emphasize the possibility that the beta-sheet of NTX is implicated in the capacity of NTX to recognize voltage-dependent K+ channels.
Determination of the three-dimensional solution structure of noxiustoxin: analysis of structural differences with related short-chain scorpion toxins.,Dauplais M, Gilquin B, Possani LD, Gurrola-Briones G, Roumestand C, Menez A Biochemistry. 1995 Dec 26;34(51):16563-73. PMID:8527429<ref>PMID:8527429</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1sxm" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Centruroides noxius]]
[[Category: Centruroides noxius]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Dauplais, M]]
[[Category: Dauplais M]]
[[Category: Gilquin, B]]
[[Category: Gilquin B]]
[[Category: Gurrola-Briones, G]]
[[Category: Gurrola-Briones G]]
[[Category: Menez, A]]
[[Category: Menez A]]
[[Category: Possani, L D]]
[[Category: Possani LD]]
[[Category: Roumestand, C]]
[[Category: Roumestand C]]
[[Category: Toxin]]

Latest revision as of 08:36, 1 May 2024

SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)

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